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Literature summary for 2.4.1.38 extracted from

  • Namdjou, D.J.; Chen, H.M.; Vinogradov, E.; Brochu, D.; Withers, S.G.; Wakarchuk, W.W.
    A beta-1,4-galactosyltransferase from Helicobacter pylori is an efficient and versatile biocatalyst displaying a novel activity for thioglycoside synthesis (2008), Chembiochem, 9, 1632-1640.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme is useful as catalyst for quantitative synthesis of Gal-beta-1,4-Man-pNP, overview Neisseria meningitidis
synthesis the enzyme is useful as catalyst for quantitative synthesis of the thiodisaccharide Gal-betaS-1,4-GlcNAcpNP as well as Gal-beta-1,4-Man-pNP, overview Helicobacter pylori

Cloned(Commentary)

Cloned (Comment) Organism
expression of full-length HP0826 in Escherichia coli Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Neisseria meningitidis
additional information
-
additional information kinetics Helicobacter pylori
0.27
-
UDP-Gal pH 7.2 Helicobacter pylori
0.79
-
pNP-beta-GlcNAc pH 7.2 Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Helicobacter pylori HP0826 is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori ?
-
?
additional information Neisseria meningitidis LgtB is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori ?
-
?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-
Neisseria meningitidis
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
160
-
purified recombinant enzyme Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information HP0826 is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori Helicobacter pylori ?
-
?
additional information LgtB is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori Neisseria meningitidis ?
-
?
additional information HP0826 exhibits a pronounced acceptor promiscuity with the most striking result being the successful transfer of Gal to the 4-SH of pNP-4-S-beta-GlcNAc Helicobacter pylori ?
-
?
UDP-Gal + 4-nitrophenyl 2-acetamido-2-deoxy-4-thio-beta-D-glucopyranoside HP0826 catalyzes the synthesis of the thiodisaccharide Gal-beta-S-1,4-GlcNAcpNP as well as Gal-beta-1,4-Man-pNP with total regio- and stereoselectivity, overview Helicobacter pylori UDP + Gal-beta-S-1,4-GlcNAcpNP
-
?
UDP-Gal + 4-nitrophenyl beta-D-mannopyranoside HP0826 catalyzes the synthesis of the thiodisaccharide Gal-beta-S-1,4-GlcNAcpNP as well as Gal-beta-1,4-Man-pNP with total regio- and stereoselectivity, overview Helicobacter pylori UDP + Gal-beta-1,4-Man-pNP
-
?
UDP-Gal + 4-nitrophenyl beta-D-mannopyranoside LgtB catalyzes the synthesis of Gal-beta-1,4-Man-pNP, overview Neisseria meningitidis UDP + Gal-beta-1,4-Man-pNP
-
?
UDP-Gal + 4-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside
-
Helicobacter pylori UDP + ?
-
?
UDP-galactose + p-nitrophenyl-beta-D-N-acetylglucosaminide
-
Helicobacter pylori ?
-
?

Synonyms

Synonyms Comment Organism
beta-1,4-galactosyltransferase
-
Neisseria meningitidis
beta-1,4-galactosyltransferase
-
Helicobacter pylori
HP0826
-
Helicobacter pylori
LgtB
-
Neisseria meningitidis
More HP0826 belongs to the carbohydrate active enzyme family GT25 of inverting glycosyltransferases Helicobacter pylori
More LgtB belongs to the carbohydrate active enzyme family GT25 of inverting glycosyltransferases Neisseria meningitidis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Helicobacter pylori