Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | required, best at 2 mM | Dictyostelium discoideum | |
additional information | not affected by Tween 20 at 0.1-1.0% | Dictyostelium discoideum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition | Dictyostelium discoideum | |
Fucalpha(1,2)Galbeta1-benzyl | competitive, 37% and 61% inhibition at 1 mM and 4 mM, respectively | Dictyostelium discoideum | |
additional information | not affected by Tween 20 at 0.1-1.0% | Dictyostelium discoideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, hyperbolic dependence on UDP-Gal concentration | Dictyostelium discoideum | |
0.0035 | - |
UDP-Gal | pH 7.4, 22°C | Dictyostelium discoideum | |
0.006 | - |
Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-Skp1 protein | pH 7.4, 22°C | Dictyostelium discoideum | |
0.82 | - |
Fucalpha(1,2)Galbeta-1-(4-nitrophenol) | pH 7.4, 22°C | Dictyostelium discoideum | |
0.84 | - |
Fucalpha(1,2)Galbeta1-benzyl | pH 7.4, 22°C | Dictyostelium discoideum | |
1.2 | - |
Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-(4-nitrophenol) | pH 7.4, 22°C | Dictyostelium discoideum | |
2.2 | - |
Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-benzyl | pH 7.4, 22°C | Dictyostelium discoideum | |
3.7 | - |
Fucalpha-1-(4-nitrophenol) | pH 7.4, 22°C | Dictyostelium discoideum | |
3.8 | - |
Fucalpha-1-benzyl | pH 7.4, 22°C | Dictyostelium discoideum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Dictyostelium discoideum | 5737 | - |
additional information | no activity in microsomes | Dictyostelium discoideum | - |
- |
soluble | - |
Dictyostelium discoideum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | lower activity compared to NaCl, best at 50 mM | Dictyostelium discoideum | |
Mg2+ | 4fold lower activity compared to Mn2+ | Dictyostelium discoideum | |
Mn2+ | required, best at 2mM | Dictyostelium discoideum | |
NaCl | preferred salt, best at 50 mM | Dictyostelium discoideum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-galactose + fucosylated Skp1 protein | Dictyostelium discoideum | - |
UDP + alpha-D-galactosyl(1-3)-fucosyl-Skp1 protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | - |
Skp1B overexpressing strain HW302 | - |
Purification (Comment) | Organism |
---|---|
native enzyme 2400fold by 2 steps ion exchange chromatography, HI chromatography, and gel filtration to homogeneity | Dictyostelium discoideum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Dictyostelium discoideum |
72 | - |
purified native enzyme | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity, enzyme forms Galalpha1,3Fuc-linkages, specific for L-fucose residues | Dictyostelium discoideum | ? | - |
? | |
UDP-D-galactose + Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-(4-nitrophenol) | - |
Dictyostelium discoideum | UDP + alpha-D-Gal-1,3-Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-(4-nitrophenol) | - |
? | |
UDP-D-galactose + Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-benzyl | - |
Dictyostelium discoideum | UDP + alpha-D-Gal(1,3)Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-benzyl | - |
? | |
UDP-D-galactose + Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-Skp1 protein | best substrate, Skp1A1-(HW120)-myc and Skp1 from strain HL250 | Dictyostelium discoideum | UDP + alpha-D-Gal(1,3)Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-Skp1 protein | - |
? | |
UDP-D-galactose + Fucalpha(1,2)Galbeta-1-(4-nitrophenol) | - |
Dictyostelium discoideum | UDP + alpha-D-Gal(1,3)Fucalpha(1,2)Galbeta-1-(4-nitrophenol) | - |
? | |
UDP-D-galactose + Fucalpha(1,2)Galbeta-1-benzyl | - |
Dictyostelium discoideum | UDP + alpha-D-Gal(1,3)Fucalpha(1,2)Galbeta-1-benzyl | - |
? | |
UDP-D-galactose + Fucalpha-1-(4-nitrophenol) | - |
Dictyostelium discoideum | UDP + alpha-D-Gal(1,3)Fucalpha-1-(4-nitrophenol) | - |
? | |
UDP-D-galactose + Fucalpha-1-benzyl | - |
Dictyostelium discoideum | UDP + alpha-D-Gal(1,3)Fucalpha-1-benzyl | - |
? | |
UDP-D-galactose + fucosylated Skp1 protein | - |
Dictyostelium discoideum | UDP + alpha-D-galactosyl(1-3)-fucosyl-Skp1 protein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alphaGal-T1 | - |
Dictyostelium discoideum |
UDP-galactose:fucoside alpha1,3-galactosyltransferase | - |
Dictyostelium discoideum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
- |
Dictyostelium discoideum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 7.4 | dependent on the buffer system | Dictyostelium discoideum |