Cloned (Comment) | Organism |
---|---|
P234S-mutant expressed in Escherichia coli BL21 | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
P234S-mutant, 1.55 and 1.65 A resolution, with and without H-antigen | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
P234S | dramatic and complete reversal of donor specificity, it preferentially utilizes UDP-GalNac for transfer | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3 | - |
Homo sapiens | |
0.088 | - |
UDP-galactose | - |
Homo sapiens | |
0.106 | - |
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3 | P234S mutant | Homo sapiens | |
4.5 | - |
UDP-galactose | P234S mutant | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P16442 | only four amino acids are responsible for B and A blood group enzymes R176G, G235S, L266M and G268A, the latter two amino acids are responsible for the difference in donor specificity and the first two have roles in acceptor binding and turnover | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + glycoprotein alpha-L-fucosyl-1,2-D-galactose | - |
Homo sapiens | UDP + glycoprotein alpha-D-galactosyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose | - |
? | |
UDP-galactose + L-fucosyl-alpha-1,2-beta-D-galactosyl-O(CH2)7CH3 | - |
Homo sapiens | UDP + alpha-D-galactosyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-galactosyl-O(CH2)7CH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GTB | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
UDP-galactose | P234S mutant | Homo sapiens | |
0.085 | - |
UDP-galactose | - |
Homo sapiens |