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Literature summary for 2.4.1.362 extracted from

  • Vuillemin, M.; Claverie, M.; Brison, Y.; Severac, E.; Bondy, P.; Morel, S.; Monsan, P.; Moulis, C.; Remaud-Simeon, M.
    Characterization of the first alpha-(1->3) branching sucrases of the GH70 family (2016), J. Biol. Chem., 291, 7687-7702 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.362

Application

Application Comment Organism
synthesis using a suitable sucrose/dextran ratio, a comb-like dextran with 50% ofx02alpha-(1->3) branching can be synthesized Leuconostoc citreum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Leuconostoc citreum

Protein Variants

Protein Variants Comment Organism
additional information expression of a truncated form deleted of the signal peptide and all APY motifs at the C-terminal end, for purification purpose Leuconostoc citreum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7
-
 sucrose no cosubstrate, pH 5.8, 30°C Leuconostoc citreum
112
-
 Dextran pH 5.8, 30°C Leuconostoc citreum

Organism

Organism UniProt Comment Textmining
Leuconostoc citreum
-
-
-
Leuconostoc citreum NRRL B-742
-
-
-

Purification (Commentary)

Purification (Comment) Organism
truncated protein variant Leuconostoc citreum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose
-
 Leuconostoc citreum D-glucose + leucrose
-
 ?
sucrose
-
 Leuconostoc citreum NRRL B-742 D-glucose + leucrose
-
 ?
sucrose + dextran dextran of 70 kDa Leuconostoc citreum D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
 ?
sucrose + dextran dextran of 70 kDa Leuconostoc citreum NRRL B-742 D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
 ?

Synonyms

Synonyms Comment Organism
branching sucrase B
-
 Leuconostoc citreum
BrsB
-
 Leuconostoc citreum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35
-
 Leuconostoc citreum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
81.4
-
 sucrose no cosubstrate, pH 5.8, 30°C Leuconostoc citreum
949
-
 sucrose cosubstrate dextran, pH 5.8, 30°C Leuconostoc citreum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
 Leuconostoc citreum