Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Dextran | presence of dextran highly stimulates the activity of domain CD2 | Apilactobacillus kunkeei |
Cloned (Comment) | Organism |
---|---|
expression of domain CD2, amino acid residues 1212-2264 of the enzyme | Apilactobacillus kunkeei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
126.7 | - |
sucrose | pH 5.5, 30°C | Apilactobacillus kunkeei | |
251.6 | - |
Dextran | pH 5.5, 30°C | Apilactobacillus kunkeei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apilactobacillus kunkeei | - |
- |
- |
Apilactobacillus kunkeei DSM 12361 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34.3 | - |
transferase activity, pH 5.5, 30°C | Apilactobacillus kunkeei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | domain CD2 additionally displays hydrolytic acivity on dextran | Apilactobacillus kunkeei | ? | - |
? | |
additional information | enzyme harbors two separate catalytic cores (CD1 and CD2), predicted to have glucansucrase and branching sucrase specificity, respectively. CD2 displays an efficient transglycosidase activity with sucrose as donor substrate. The CD2-catalyzed transglycosylation reaction follows a Ping Pong Bi Bi mechanism and produces single glucosyl (alpha1->3) linked branches onto dextran, resulting in the production of highly branched comb-like alpha-glucan products | Apilactobacillus kunkeei | ? | - |
? | |
additional information | domain CD2 additionally displays hydrolytic acivity on dextran | Apilactobacillus kunkeei DSM 12361 | ? | - |
? | |
additional information | enzyme harbors two separate catalytic cores (CD1 and CD2), predicted to have glucansucrase and branching sucrase specificity, respectively. CD2 displays an efficient transglycosidase activity with sucrose as donor substrate. The CD2-catalyzed transglycosylation reaction follows a Ping Pong Bi Bi mechanism and produces single glucosyl (alpha1->3) linked branches onto dextran, resulting in the production of highly branched comb-like alpha-glucan products | Apilactobacillus kunkeei DSM 12361 | ? | - |
? | |
sucrose + dextran | dextran of 70 kDa | Apilactobacillus kunkeei | D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch | - |
? | |
sucrose + dextran | dextran of 70 kDa | Apilactobacillus kunkeei DSM 12361 | D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 130000, isolated domain CD2, SDS-PAGE, x * 121000, isolated domain CD2, calculated from sequence | Apilactobacillus kunkeei |
Synonyms | Comment | Organism |
---|---|---|
GtfZ | - |
Apilactobacillus kunkeei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
isolated domain CD2 | Apilactobacillus kunkeei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
8 h, 90% residual activity, isolated domain CD2 | Apilactobacillus kunkeei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
isolated domain CD2 | Apilactobacillus kunkeei |