Activating Compound | Comment | Organism | Structure |
---|---|---|---|
heparin | activates | Propionibacterium freudenreichii |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Propionibacterium freudenreichii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Propionibacterium freudenreichii | |
NaCl | an increase in the concentration of NaCl from 0 to 100 mM leads to a decrease in the OtsA activity by more than 35% when ADP-glucose or TDP-glucose is the substrate. When UDP-glucose or GDP-glucose is used as substrate, the OtsA activity is increased by 10-30% | Propionibacterium freudenreichii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
UDP-glucose | 37°C, pH 5.5 | Propionibacterium freudenreichii | |
0.4 | - |
GDP-glucose | 37°C, pH 5.5 | Propionibacterium freudenreichii | |
0.6 | - |
ADP-glucose | 37°C, pH 5.5 | Propionibacterium freudenreichii | |
2 | - |
TDP-glucose | 37°C, pH 5.5 | Propionibacterium freudenreichii | |
3 | - |
D-glucose 6-phosphate | 37°C, pH 5.5, cosubstrate: UDP-glucose | Propionibacterium freudenreichii | |
4.2 | - |
D-glucose 6-phosphate | 37°C, pH 5.5, cosubstrate: ADP-glucose | Propionibacterium freudenreichii | |
5.3 | - |
D-glucose 6-phosphate | 37°C, pH 5.5, cosubstrate: TDP-glucose | Propionibacterium freudenreichii | |
7.1 | - |
D-glucose 6-phosphate | 37°C, pH 5.5, cosubstrate: GDP-glucose | Propionibacterium freudenreichii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | upon an increase in the KCl concentration from 0 to 100 mM, the OtsA activity decreases by more than 40%, whereas it is not significantly affected when UDP or GDP-glucose is used as the substrate | Propionibacterium freudenreichii | |
Mg2+ | 10 mM, required for maximal activity | Propionibacterium freudenreichii | |
NaCl | an increase in the concentration of NaCl from 0 to 100 mM leads to a decrease in the OtsA activity by more than 35% when ADP-glucose or TDP-glucose is the substrate. When UDP-glucose or GDP-glucose is used as substrate, the OtsA activity is increased by 10-30% | Propionibacterium freudenreichii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Propionibacterium freudenreichii | the trehalose-6-phosphate synthase/phosphatase (OtsA-OtsB) pathway plays an important role in the synthesis of trehalose in response to stress | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Propionibacterium freudenreichii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Propionibacterium freudenreichii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-alpha-D-glucose + D-glucose 6-phosphate | in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract | Propionibacterium freudenreichii | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
GDP-alpha-D-glucose + D-glucose 6-phosphate | in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract | Propionibacterium freudenreichii | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
additional information | the trehalose-6-phosphate synthase/phosphatase (OtsA-OtsB) pathway plays an important role in the synthesis of trehalose in response to stress | Propionibacterium freudenreichii | ? | - |
? | |
TDP-alpha-D-glucose + D-glucose 6-phosphate | in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract | Propionibacterium freudenreichii | TDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract | Propionibacterium freudenreichii | UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OtsA | - |
Propionibacterium freudenreichii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Propionibacterium freudenreichii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
activity with ADP-glucose, UDP-glucose and TDP-glucose | Propionibacterium freudenreichii |
5.5 | - |
activitry with GDP-glucose | Propionibacterium freudenreichii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | pH 5.0: about 85% of maximal activity, pH 6.0: about 50% of maximal activity | Propionibacterium freudenreichii |