Literature summary for 2.4.1.345 extracted from

Guerin, M.E.; Buschiazzo, A.; Kordulakova, J.; Jackson, M.; Alzari, P.M.
Crystallization and preliminary crystallographic analysis of PimA, an essential mannosyltransferase from Mycobacterium smegmatis (2005), Acta Crystallogr. Sect. F, 61, 518-520.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Mycolicibacterium smegmatis
expression in Escherichia coli BL21(DE3)pLysS cells transformed wth pET-pimA	Mycolicibacterium smegmatis

Crystallization (Commentary)

Crystallization (Comment)	Organism
in the presence of GDP and myo-inositol, to 2.4 A resolution, orthorhombic space group P212121, one molecule per asymmetric unit	Mycolicibacterium smegmatis
sitting-drop vapour-diffusion method. The crystals belong to space group P2(1)2(1)2(1) with unit-cell parameters a = 37.2, b = 72.4, c = 138.2 A and diffract to 2.4 A resolution	Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
43300	-	-	Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Mycolicibacterium smegmatis	essential enzyme for mycobacterial growth	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	-	-	-
Mycolicibacterium smegmatis	A0QWG6	-	-
Mycolicibacterium smegmatis ATCC 700084	A0QWG6	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	essential enzyme for mycobacterial growth	Mycolicibacterium smegmatis	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 43300, SDS-PAGE	Mycolicibacterium smegmatis

Synonyms

Synonyms	Comment	Organism
phosphatidylinositol mannosyltransferase	-	Mycolicibacterium smegmatis
PimA	-	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)