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Literature summary for 2.4.1.340 extracted from

  • Chiku, K.; Nihira, T.; Suzuki, E.; Nishimoto, M.; Kitaoka, M.; Ohtsubo, K.; Nakai, H.
    Discovery of two beta-1,2-mannoside phosphorylases showing different chain-length specificities from Thermoanaerobacter sp. X-514 (2014), PLoS One, 9, e114882.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in in Escherichia coli Rosetta 2 (DE3) Thermoanaerobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
D-fructose pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
0.41
-
alpha-D-mannose pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
0.52
-
[(1->2)-beta-D-mannosyl]2 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
0.63
-
phosphate cosubstrate: [(1->2)-beta-D-mannosyl]4, pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
0.71
-
phosphate cosubstrate: [(1->2)-beta-D-mannosyl]3, pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
0.74
-
phosphate cosubstrate: [(1->2)-beta-D-mannosyl]2, pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
0.77
-
[(1->2)-beta-D-mannosyl]2 pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
0.92
-
[(1->2)-beta-D-mannosyl]3 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
1.2
-
[(1->2)-beta-D-mannosyl]3 pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
1.4
-
alpha-D-mannose 1-phosphate pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2.3
-
[(1->2)-beta-D-mannosyl]4 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33997
-
2 * 33997, calculated from sequence Thermoanaerobacter sp.
34000
-
2 * 34000, SDS-PAGE Thermoanaerobacter sp.
50000
-
gel filtration Thermoanaerobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[(1->2)-beta-D-mannosyl]n + phosphate Thermoanaerobacter sp. involved in GDP-D-mannose biosynthesis [(1->2)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter sp. B0K2C2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermoanaerobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-mannose + alpha-D-mannose 1-phosphate in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues Thermoanaerobacter sp. [(1->2)-beta-D-mannosyl]2 + phosphate
-
?
D-fructose + alpha-D-mannose 1-phosphate
-
Thermoanaerobacter sp. ? + phosphate
-
?
[(1->2)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues Thermoanaerobacter sp. [(1->2)-beta-D-mannosyl]3 + phosphate
-
?
[(1->2)-beta-D-mannosyl]2 + phosphate in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues Thermoanaerobacter sp. alpha-D-mannose 1-phosphate
-
r
[(1->2)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues Thermoanaerobacter sp. [(1->2)-beta-D-mannosyl]4 + phosphate
-
?
[(1->2)-beta-D-mannosyl]3 + phosphate in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues Thermoanaerobacter sp. [(1->2)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate
-
r
[(1->2)-beta-D-mannosyl]4 + alpha-D-mannose 1-phosphate in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues Thermoanaerobacter sp. [(1->2)-beta-D-mannosyl]5 + phosphate
-
?
[(1->2)-beta-D-mannosyl]4 + phosphate in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues Thermoanaerobacter sp. [(1->2)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate
-
r
[(1->2)-beta-D-mannosyl]n + phosphate involved in GDP-D-mannose biosynthesis Thermoanaerobacter sp. [(1->2)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
-
r

Subunits

Subunits Comment Organism
homodimer 2 * 34000, SDS-PAGE Thermoanaerobacter sp.
homodimer 2 * 33997, calculated from sequence Thermoanaerobacter sp.

Synonyms

Synonyms Comment Organism
1,2-beta-oligomannan phosphorylase
-
Thermoanaerobacter sp.
1,2-beta-oligomannan:phosphate alpha-D-mannosyltransferase
-
Thermoanaerobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
stable up to 55°C during a 30 min incubation Thermoanaerobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
[(1->2)-beta-D-mannosyl]2 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
1.9
-
alpha-D-mannose pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
2
-
D-fructose pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
20
-
[(1->2)-beta-D-mannosyl]3 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
22
-
alpha-D-mannose 1-phosphate pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
25
-
[(1->2)-beta-D-mannosyl]2 pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
43
-
[(1->2)-beta-D-mannosyl]3 pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
49
-
[(1->2)-beta-D-mannosyl]4 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
synthetic reaction Thermoanaerobacter sp.
6
-
phosphorolytic activity Thermoanaerobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9.5 stable in pH-range 4.0-9.5 during a 30 min incubation Thermoanaerobacter sp.

General Information

General Information Comment Organism
metabolism involved in GDP-D-mannose biosynthesis Thermoanaerobacter sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.7
-
[(1->2)-beta-D-mannosyl]2 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
4.6
-
alpha-D-mannose pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
10
-
D-fructose pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
16
-
alpha-D-mannose 1-phosphate pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
21
-
[(1->2)-beta-D-mannosyl]4 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
22
-
[(1->2)-beta-D-mannosyl]3 pH 5.0, 30°C, phosphorolytic reaction Thermoanaerobacter sp.
32
-
[(1->2)-beta-D-mannosyl]2 pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.
36
-
[(1->2)-beta-D-mannosyl]3 pH 5.0, 30°C, synthetic reaction Thermoanaerobacter sp.