Cloned (Comment) | Organism |
---|---|
expression in in Escherichia coli Rosetta 2 (DE3) | Thermoanaerobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
D-fructose | pH 5.0, 30°C, synthetic reaction | Thermoanaerobacter sp. | |
0.41 | - |
alpha-D-mannose | pH 5.0, 30°C, synthetic reaction | Thermoanaerobacter sp. | |
0.52 | - |
[(1->2)-beta-D-mannosyl]2 | pH 5.0, 30°C, phosphorolytic reaction | Thermoanaerobacter sp. | |
0.63 | - |
phosphate | cosubstrate: [(1->2)-beta-D-mannosyl]4, pH 5.0, 30°C, phosphorolytic reaction | Thermoanaerobacter sp. | |
0.71 | - |
phosphate | cosubstrate: [(1->2)-beta-D-mannosyl]3, pH 5.0, 30°C, phosphorolytic reaction | Thermoanaerobacter sp. | |
0.74 | - |
phosphate | cosubstrate: [(1->2)-beta-D-mannosyl]2, pH 5.0, 30°C, phosphorolytic reaction | Thermoanaerobacter sp. | |
0.77 | - |
[(1->2)-beta-D-mannosyl]2 | pH 5.0, 30°C, synthetic reaction | Thermoanaerobacter sp. | |
0.92 | - |
[(1->2)-beta-D-mannosyl]3 | pH 5.0, 30°C, phosphorolytic reaction | Thermoanaerobacter sp. | |
1.2 | - |
[(1->2)-beta-D-mannosyl]3 | pH 5.0, 30°C, synthetic reaction | Thermoanaerobacter sp. | |
1.4 | - |
alpha-D-mannose 1-phosphate | pH 5.0, 30°C, synthetic reaction | Thermoanaerobacter sp. | |
2.3 | - |
[(1->2)-beta-D-mannosyl]4 | pH 5.0, 30°C, phosphorolytic reaction | Thermoanaerobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33997 | - |
2 * 33997, calculated from sequence | Thermoanaerobacter sp. |
34000 | - |
2 * 34000, SDS-PAGE | Thermoanaerobacter sp. |
50000 | - |
gel filtration | Thermoanaerobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(1->2)-beta-D-mannosyl]n + phosphate | Thermoanaerobacter sp. | involved in GDP-D-mannose biosynthesis | [(1->2)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter sp. | B0K2C2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoanaerobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-mannose + alpha-D-mannose 1-phosphate | in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues | Thermoanaerobacter sp. | [(1->2)-beta-D-mannosyl]2 + phosphate | - |
? | |
D-fructose + alpha-D-mannose 1-phosphate | - |
Thermoanaerobacter sp. | ? + phosphate | - |
? | |
[(1->2)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate | in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues | Thermoanaerobacter sp. | [(1->2)-beta-D-mannosyl]3 + phosphate | - |
? | |
[(1->2)-beta-D-mannosyl]2 + phosphate | in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues | Thermoanaerobacter sp. | alpha-D-mannose 1-phosphate | - |
r | |
[(1->2)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate | in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues | Thermoanaerobacter sp. | [(1->2)-beta-D-mannosyl]4 + phosphate | - |
? | |
[(1->2)-beta-D-mannosyl]3 + phosphate | in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues | Thermoanaerobacter sp. | [(1->2)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate | - |
r | |
[(1->2)-beta-D-mannosyl]4 + alpha-D-mannose 1-phosphate | in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues | Thermoanaerobacter sp. | [(1->2)-beta-D-mannosyl]5 + phosphate | - |
? | |
[(1->2)-beta-D-mannosyl]4 + phosphate | in the synthetic direction it produces oligosaccharides with a degree of polymerization of 3, 4 and 5. The phosphorolysis reaction proceeds to completion, although activity is highest when the substrate has at least three residues | Thermoanaerobacter sp. | [(1->2)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate | - |
r | |
[(1->2)-beta-D-mannosyl]n + phosphate | involved in GDP-D-mannose biosynthesis | Thermoanaerobacter sp. | [(1->2)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 34000, SDS-PAGE | Thermoanaerobacter sp. |
homodimer | 2 * 33997, calculated from sequence | Thermoanaerobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
1,2-beta-oligomannan phosphorylase | - |
Thermoanaerobacter sp. |
1,2-beta-oligomannan:phosphate alpha-D-mannosyltransferase | - |
Thermoanaerobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
stable up to 55°C during a 30 min incubation | Thermoanaerobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
[(1->2)-beta-D-mannosyl]2 | pH 5.0, 30°C, phosphorolytic reaction | Thermoanaerobacter sp. | |
1.9 | - |
alpha-D-mannose | pH 5.0, 30°C, synthetic reaction | Thermoanaerobacter sp. | |
2 | - |
D-fructose | pH 5.0, 30°C, synthetic reaction | Thermoanaerobacter sp. | |
20 | - |
[(1->2)-beta-D-mannosyl]3 | pH 5.0, 30°C, phosphorolytic reaction | Thermoanaerobacter sp. | |
22 | - |
alpha-D-mannose 1-phosphate | pH 5.0, 30°C, synthetic reaction | Thermoanaerobacter sp. | |
25 | - |
[(1->2)-beta-D-mannosyl]2 | pH 5.0, 30°C, synthetic reaction | Thermoanaerobacter sp. | |
43 | - |
[(1->2)-beta-D-mannosyl]3 | pH 5.0, 30°C, synthetic reaction | Thermoanaerobacter sp. | |
49 | - |
[(1->2)-beta-D-mannosyl]4 | pH 5.0, 30°C, phosphorolytic reaction | Thermoanaerobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
synthetic reaction | Thermoanaerobacter sp. |
6 | - |
phosphorolytic activity | Thermoanaerobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9.5 | stable in pH-range 4.0-9.5 during a 30 min incubation | Thermoanaerobacter sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | involved in GDP-D-mannose biosynthesis | Thermoanaerobacter sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
[(1->2)-beta-D-mannosyl]2 | pH 5.0, 30°C, phosphorolytic reaction | Thermoanaerobacter sp. | |
4.6 | - |
alpha-D-mannose | pH 5.0, 30°C, synthetic reaction | Thermoanaerobacter sp. | |
10 | - |
D-fructose | pH 5.0, 30°C, synthetic reaction | Thermoanaerobacter sp. | |
16 | - |
alpha-D-mannose 1-phosphate | pH 5.0, 30°C, synthetic reaction | Thermoanaerobacter sp. | |
21 | - |
[(1->2)-beta-D-mannosyl]4 | pH 5.0, 30°C, phosphorolytic reaction | Thermoanaerobacter sp. | |
22 | - |
[(1->2)-beta-D-mannosyl]3 | pH 5.0, 30°C, phosphorolytic reaction | Thermoanaerobacter sp. | |
32 | - |
[(1->2)-beta-D-mannosyl]2 | pH 5.0, 30°C, synthetic reaction | Thermoanaerobacter sp. | |
36 | - |
[(1->2)-beta-D-mannosyl]3 | pH 5.0, 30°C, synthetic reaction | Thermoanaerobacter sp. |