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Literature summary for 2.4.1.333 extracted from
- Taguchi, H.; Kitaoka, M.: 1,2-beta-Oligoglucan phosphorylase from Listeria innocua (2014), PLoS ONE, 9, e92353.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 | Listeria innocua |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose | pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate | Listeria innocua |  |
| 1.2 | - |
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose | pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
| 1.2 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 30°C, cosubstrate sophorose | Listeria innocua | |
| 1.8 | - |
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose | pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
| 6 | - |
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose | pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
| 6.8 | - |
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose | pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
| 8.5 | - |
sophorose | pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Listeria innocua | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
1 * 120000, SDS-PAGE | Listeria innocua |
| 122900 | - |
calculated from amino acid sequence | Listeria innocua |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [(1->2)-beta-D-glucosyl]n + phosphate | Listeria innocua | the enzyme catalyses reversible the phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39 | [(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| [(1->2)-beta-D-glucosyl]n + phosphate | Listeria innocua CLIP 11262 | the enzyme catalyses reversible the phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39 | [(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| [(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | Listeria innocua | the average product shows a polymerisation degree of 39 glucose units | [(1->2)-beta-D-glucosyl]n + phosphate | - |
r | |
| [(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | Listeria innocua CLIP 11262 | the average product shows a polymerisation degree of 39 glucose units | [(1->2)-beta-D-glucosyl]n + phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Listeria innocua | Q92AT0 | - |
- |
| Listeria innocua CLIP 11262 | Q92AT0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Listeria innocua |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate | - |
Listeria innocua | 2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucopyranose 1-phosphate | - |
? | |
| 2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate | - |
Listeria innocua CLIP 11262 | 2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucopyranose 1-phosphate | - |
? | |
| 2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate | - |
Listeria innocua | 2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucopyranose 1-phosphate | - |
? | |
| 2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate | - |
Listeria innocua | 2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate | - |
r | |
| 2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate | - |
Listeria innocua | sophorose + alpha-D-glucopyranose 1-phosphate | - |
ir | |
| 2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate | - |
Listeria innocua | sophorose + alpha-D-glucose 1-phosphate | - |
ir | |
| sophorose + alpha-D-glucose 1-phosphate | - |
Listeria innocua | 2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate | - |
r | |
| [(1->2)-beta-D-glucosyl]n + phosphate | the enzyme catalyses reversible the phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39 | Listeria innocua | [(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| [(1->2)-beta-D-glucosyl]n + phosphate | the enzyme catalyses the reversible phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39 | Listeria innocua | [(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| [(1->2)-beta-D-glucosyl]n + phosphate | the enzyme catalyses reversible the phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39 | Listeria innocua CLIP 11262 | [(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| [(1->2)-beta-D-glucosyl]n + phosphate | the enzyme catalyses the reversible phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39 | Listeria innocua CLIP 11262 | [(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| [(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | the average product shows a polymerisation degree of 39 glucose units | Listeria innocua | [(1->2)-beta-D-glucosyl]n + phosphate | - |
r | |
| [(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | the enzyme catalyses the reversible phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39 | Listeria innocua | [(1->2)-beta-D-glucosyl]n + phosphate | - |
r | |
| [(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | the average product shows a polymerisation degree of 39 glucose units | Listeria innocua CLIP 11262 | [(1->2)-beta-D-glucosyl]n + phosphate | - |
r | |
| [(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | the enzyme catalyses the reversible phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39 | Listeria innocua CLIP 11262 | [(1->2)-beta-D-glucosyl]n + phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 120000, SDS-PAGE | Listeria innocua |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lin1839 | locus name | Listeria innocua |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19 | - |
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose | pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
| 30 | - |
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose | pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
| 31 | - |
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose | pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
| 43 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 30°C, cosubstrate sophorose | Listeria innocua | |
| 90 | - |
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose | pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
| 97 | - |
sophorose | pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
| 110 | - |
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose | pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11 | - |
sophorose | pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
| 13 | - |
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose | pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
| 17 | - |
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose | pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
| 18 | - |
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose | pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
| 19 | - |
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose | pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
| 24 | - |
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose | pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate | Listeria innocua | |
| 34 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 30°C, cosubstrate sophorose | Listeria innocua | |
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