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Literature summary for 2.4.1.332 extracted from

  • Nihira, T.; Saito, Y.; Ohtsubo, K.-i.; , Nakai1, H.; Kitaoka, M.
    2-O-alpha-D-Glucosylglycerol phosphorylase from Bacillus selenitireducens MLS10 possessing hydrolytic activity on beta-D-glucose 1-phosphate (2014), PLoS One, 9, e86548.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Salisediminibacterium selenitireducens

Protein Variants

Protein Variants Comment Organism
E475A inactive mutant Salisediminibacterium selenitireducens
E475Q inactive mutant Salisediminibacterium selenitireducens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
beta-D-glucose 1-phosphate the Km for beta-D-glucose 1-phosphate in the hydrolytic reaction is 0.041 and 0.16 mM at pH 6.0 and pH 7.5 respectively Salisediminibacterium selenitireducens
0.45
-
beta-D-glucose 1-phosphate pH 7.5, 30°C Salisediminibacterium selenitireducens
0.57
-
phosphate pH 8.0, 30°C Salisediminibacterium selenitireducens
1.1
-
2-O-alpha-D-glucopyranosyl-sn-glycerol pH 8.0, 30°C Salisediminibacterium selenitireducens
5.7
-
sn-glycerol pH 7.5, 30°C Salisediminibacterium selenitireducens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86260
-
calculated from amino acid sequence Salisediminibacterium selenitireducens
87000
-
2 * 87000, SDS-PAGE Salisediminibacterium selenitireducens
174000
-
gel filtration Salisediminibacterium selenitireducens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate Salisediminibacterium selenitireducens
-
beta-D-glucose 1-phosphate + sn-glycerol
-
r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate Salisediminibacterium selenitireducens DSM 15326
-
beta-D-glucose 1-phosphate + sn-glycerol
-
r
beta-D-glucose 1-phosphate + sn-glycerol Salisediminibacterium selenitireducens
-
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
r
beta-D-glucose 1-phosphate + sn-glycerol Salisediminibacterium selenitireducens DSM 15326
-
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
r

Organism

Organism UniProt Comment Textmining
Salisediminibacterium selenitireducens D6XZ22
-
-
Salisediminibacterium selenitireducens DSM 15326 D6XZ22
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Salisediminibacterium selenitireducens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
Salisediminibacterium selenitireducens beta-D-glucose 1-phosphate + sn-glycerol
-
r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
Salisediminibacterium selenitireducens DSM 15326 beta-D-glucose 1-phosphate + sn-glycerol
-
r
beta-D-glucose 1-phosphate + sn-glycerol
-
Salisediminibacterium selenitireducens 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
?
beta-D-glucose 1-phosphate + sn-glycerol
-
Salisediminibacterium selenitireducens 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
r
beta-D-glucose 1-phosphate + sn-glycerol
-
Salisediminibacterium selenitireducens DSM 15326 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
?
beta-D-glucose 1-phosphate + sn-glycerol
-
Salisediminibacterium selenitireducens DSM 15326 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
r
additional information 1-butanol, 2-butanol, cyclohexanol, benzyl alcohol, hydroquinone, phenol, ethylene glycol, glycerol, erythritol, xylitol D-arabinitol, L-arabinitol, and D-glucitol are no substitutes for sn-glycerol. With beta-D-glucose 1-phosphate as donor the following compounds are no acceptors: D-glucose, D-mannose, D-allose, D-galactose, D-altrose, D-talose, and D-fructose, D-xylose, D-lyxose, D-ribose, D-arabinose, and L-arabinose, D-glucosamine, D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-D-mannosamine, N-acetyl-D-galactosamine, D-glucuronic acid, D-galacturonic acid, 1,5-anhydro-D-glucitol, 2-deoxy-D-glucose, L-rhamnose, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, 3-Omethyl-D-glucose, methyl-alpha-D-glucoside, and methyl-beta-D-glucoside, trehalose, kojibiose, nigerose, maltose, isomaltose, sucrose, sophorose, laminaribiose, cellobiose, gentiobiose, lactose, lactulose, melibiose, xylobiose, beta-1,4-mannobiose, N,N'-diacetylchitobiose Salisediminibacterium selenitireducens ?
-
?
additional information 1-butanol, 2-butanol, cyclohexanol, benzyl alcohol, hydroquinone, phenol, ethylene glycol, glycerol, erythritol, xylitol D-arabinitol, L-arabinitol, and D-glucitol are no substitutes for sn-glycerol. With beta-D-glucose 1-phosphate as donor the following compounds are no acceptors: D-glucose, D-mannose, D-allose, D-galactose, D-altrose, D-talose, and D-fructose, D-xylose, D-lyxose, D-ribose, D-arabinose, and L-arabinose, D-glucosamine, D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-D-mannosamine, N-acetyl-D-galactosamine, D-glucuronic acid, D-galacturonic acid, 1,5-anhydro-D-glucitol, 2-deoxy-D-glucose, L-rhamnose, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, 3-Omethyl-D-glucose, methyl-alpha-D-glucoside, and methyl-beta-D-glucoside, trehalose, kojibiose, nigerose, maltose, isomaltose, sucrose, sophorose, laminaribiose, cellobiose, gentiobiose, lactose, lactulose, melibiose, xylobiose, beta-1,4-mannobiose, N,N'-diacetylchitobiose Salisediminibacterium selenitireducens DSM 15326 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 87000, SDS-PAGE Salisediminibacterium selenitireducens

Synonyms

Synonyms Comment Organism
2-O-alpha-D-glucopyranosylglycerol phosphorylase
-
Salisediminibacterium selenitireducens
Bsel_2816 locus name Salisediminibacterium selenitireducens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
beta-D-glucose 1-phosphate kcat for beta-D-glucose 1-phosphate in the hydrolytic reaction is 2.8 and 1.7 s(-1) at pH 6.0 and pH 7.5 respectively Salisediminibacterium selenitireducens
95
-
2-O-alpha-D-glucopyranosyl-sn-glycerol pH 8.0, 30°C Salisediminibacterium selenitireducens
163
-
sn-glycerol pH 7.5, 30°C Salisediminibacterium selenitireducens
180
-
beta-D-glucose 1-phosphate pH 7.5, 30°C Salisediminibacterium selenitireducens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
hydrolysis of beta-D-glucose 1-phosphate has an optimum of pH 6.0 Salisediminibacterium selenitireducens
7.5
-
synthesis of 2-O-alpha-D-glucopyranosyl-sn-glycerol Salisediminibacterium selenitireducens
8
-
phosphorolytic activity Salisediminibacterium selenitireducens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 10.5 4°C, 24 h stable Salisediminibacterium selenitireducens