Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Salisediminibacterium selenitireducens |
Protein Variants | Comment | Organism |
---|---|---|
E475A | inactive mutant | Salisediminibacterium selenitireducens |
E475Q | inactive mutant | Salisediminibacterium selenitireducens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
beta-D-glucose 1-phosphate | the Km for beta-D-glucose 1-phosphate in the hydrolytic reaction is 0.041 and 0.16 mM at pH 6.0 and pH 7.5 respectively | Salisediminibacterium selenitireducens | |
0.45 | - |
beta-D-glucose 1-phosphate | pH 7.5, 30°C | Salisediminibacterium selenitireducens | |
0.57 | - |
phosphate | pH 8.0, 30°C | Salisediminibacterium selenitireducens | |
1.1 | - |
2-O-alpha-D-glucopyranosyl-sn-glycerol | pH 8.0, 30°C | Salisediminibacterium selenitireducens | |
5.7 | - |
sn-glycerol | pH 7.5, 30°C | Salisediminibacterium selenitireducens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86260 | - |
calculated from amino acid sequence | Salisediminibacterium selenitireducens |
87000 | - |
2 * 87000, SDS-PAGE | Salisediminibacterium selenitireducens |
174000 | - |
gel filtration | Salisediminibacterium selenitireducens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | Salisediminibacterium selenitireducens | - |
beta-D-glucose 1-phosphate + sn-glycerol | - |
r | |
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | Salisediminibacterium selenitireducens DSM 15326 | - |
beta-D-glucose 1-phosphate + sn-glycerol | - |
r | |
beta-D-glucose 1-phosphate + sn-glycerol | Salisediminibacterium selenitireducens | - |
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
r | |
beta-D-glucose 1-phosphate + sn-glycerol | Salisediminibacterium selenitireducens DSM 15326 | - |
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salisediminibacterium selenitireducens | D6XZ22 | - |
- |
Salisediminibacterium selenitireducens DSM 15326 | D6XZ22 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Salisediminibacterium selenitireducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
Salisediminibacterium selenitireducens | beta-D-glucose 1-phosphate + sn-glycerol | - |
r | |
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
Salisediminibacterium selenitireducens DSM 15326 | beta-D-glucose 1-phosphate + sn-glycerol | - |
r | |
beta-D-glucose 1-phosphate + sn-glycerol | - |
Salisediminibacterium selenitireducens | 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
? | |
beta-D-glucose 1-phosphate + sn-glycerol | - |
Salisediminibacterium selenitireducens | 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
r | |
beta-D-glucose 1-phosphate + sn-glycerol | - |
Salisediminibacterium selenitireducens DSM 15326 | 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
? | |
beta-D-glucose 1-phosphate + sn-glycerol | - |
Salisediminibacterium selenitireducens DSM 15326 | 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate | - |
r | |
additional information | 1-butanol, 2-butanol, cyclohexanol, benzyl alcohol, hydroquinone, phenol, ethylene glycol, glycerol, erythritol, xylitol D-arabinitol, L-arabinitol, and D-glucitol are no substitutes for sn-glycerol. With beta-D-glucose 1-phosphate as donor the following compounds are no acceptors: D-glucose, D-mannose, D-allose, D-galactose, D-altrose, D-talose, and D-fructose, D-xylose, D-lyxose, D-ribose, D-arabinose, and L-arabinose, D-glucosamine, D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-D-mannosamine, N-acetyl-D-galactosamine, D-glucuronic acid, D-galacturonic acid, 1,5-anhydro-D-glucitol, 2-deoxy-D-glucose, L-rhamnose, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, 3-Omethyl-D-glucose, methyl-alpha-D-glucoside, and methyl-beta-D-glucoside, trehalose, kojibiose, nigerose, maltose, isomaltose, sucrose, sophorose, laminaribiose, cellobiose, gentiobiose, lactose, lactulose, melibiose, xylobiose, beta-1,4-mannobiose, N,N'-diacetylchitobiose | Salisediminibacterium selenitireducens | ? | - |
? | |
additional information | 1-butanol, 2-butanol, cyclohexanol, benzyl alcohol, hydroquinone, phenol, ethylene glycol, glycerol, erythritol, xylitol D-arabinitol, L-arabinitol, and D-glucitol are no substitutes for sn-glycerol. With beta-D-glucose 1-phosphate as donor the following compounds are no acceptors: D-glucose, D-mannose, D-allose, D-galactose, D-altrose, D-talose, and D-fructose, D-xylose, D-lyxose, D-ribose, D-arabinose, and L-arabinose, D-glucosamine, D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-D-mannosamine, N-acetyl-D-galactosamine, D-glucuronic acid, D-galacturonic acid, 1,5-anhydro-D-glucitol, 2-deoxy-D-glucose, L-rhamnose, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, 3-Omethyl-D-glucose, methyl-alpha-D-glucoside, and methyl-beta-D-glucoside, trehalose, kojibiose, nigerose, maltose, isomaltose, sucrose, sophorose, laminaribiose, cellobiose, gentiobiose, lactose, lactulose, melibiose, xylobiose, beta-1,4-mannobiose, N,N'-diacetylchitobiose | Salisediminibacterium selenitireducens DSM 15326 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 87000, SDS-PAGE | Salisediminibacterium selenitireducens |
Synonyms | Comment | Organism |
---|---|---|
2-O-alpha-D-glucopyranosylglycerol phosphorylase | - |
Salisediminibacterium selenitireducens |
Bsel_2816 | locus name | Salisediminibacterium selenitireducens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
beta-D-glucose 1-phosphate | kcat for beta-D-glucose 1-phosphate in the hydrolytic reaction is 2.8 and 1.7 s(-1) at pH 6.0 and pH 7.5 respectively | Salisediminibacterium selenitireducens | |
95 | - |
2-O-alpha-D-glucopyranosyl-sn-glycerol | pH 8.0, 30°C | Salisediminibacterium selenitireducens | |
163 | - |
sn-glycerol | pH 7.5, 30°C | Salisediminibacterium selenitireducens | |
180 | - |
beta-D-glucose 1-phosphate | pH 7.5, 30°C | Salisediminibacterium selenitireducens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
hydrolysis of beta-D-glucose 1-phosphate has an optimum of pH 6.0 | Salisediminibacterium selenitireducens |
7.5 | - |
synthesis of 2-O-alpha-D-glucopyranosyl-sn-glycerol | Salisediminibacterium selenitireducens |
8 | - |
phosphorolytic activity | Salisediminibacterium selenitireducens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.5 | 4°C, 24 h stable | Salisediminibacterium selenitireducens |