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Literature summary for 2.4.1.327 extracted from
- AknK is an L-2-deoxyfucosyltransferase in the biosynthesis of the anthracycline aclacinomycin A (2004), Biochemistry, 43, 4548-4558.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Streptomyces galilaeus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1044 | - |
rhodosaminyl aklavinone | with TDP-L-daunosamine as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C | Streptomyces galilaeus |  |
| 0.1091 | - |
rhodosaminyl aklavinone | with TDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C | Streptomyces galilaeus | |
| 0.138 | - |
idarubicin | in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C | Streptomyces galilaeus | |
| 0.1489 | - |
dTDP-L-2-deoxyfucose | with rhodosaminyl aklavinone as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C | Streptomyces galilaeus | |
| 0.531 | - |
dTDP-L-2-deoxyfucose | with idarubicin as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C | Streptomyces galilaeus | |
| 0.94 | - |
dTDP-L-daunosamine | in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C | Streptomyces galilaeus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50685 | - |
x * 50685, calculated from amino acid sequence | Streptomyces galilaeus |
| 50699 | - |
x * 50699, MALDI-TOF mass spectrometry | Streptomyces galilaeus |
| 65000 | - |
x * 65000, His6-tagged enzyme, SDS-PAGE | Streptomyces galilaeus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTDP-2-deoxy-beta-L-fucose + aclacinomycin T | Streptomyces galilaeus | - |
dTDP + aclacinomycin S | - |
? | |
| dTDP-2-deoxy-beta-L-fucose + aclacinomycin T | Streptomyces galilaeus ATCC 31615 | - |
dTDP + aclacinomycin S | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces galilaeus | - |
- |
- |
| Streptomyces galilaeus ATCC 31615 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography | Streptomyces galilaeus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTDP-2-deoxy-beta-L-fucose + aclacinomycin T | - |
Streptomyces galilaeus | dTDP + aclacinomycin S | - |
? | |
| dTDP-2-deoxy-beta-L-fucose + aclacinomycin T | - |
Streptomyces galilaeus ATCC 31615 | dTDP + aclacinomycin S | - |
? | |
| dTDP-L-2-deoxyfucose + aclacinomycin S | - |
Streptomyces galilaeus | dTDP + 2-deoxy-alpha-D-fucosyl-aclacinomyin S | - |
? | |
| dTDP-L-2-deoxyfucose + aclacinomycin S | - |
Streptomyces galilaeus ATCC 31615 | dTDP + 2-deoxy-alpha-D-fucosyl-aclacinomyin S | - |
? | |
| dTDP-L-2-deoxyfucose + aclacinomycin T | - |
Streptomyces galilaeus | dTDP + aclacinomycin S | - |
? | |
| dTDP-L-2-deoxyfucose + aclacinomycin T | - |
Streptomyces galilaeus ATCC 31615 | dTDP + aclacinomycin S | - |
? | |
| idarubicin + dTDP-L-2-deoxyfucose | - |
Streptomyces galilaeus | dTDP + 4'-O-(alpha-L-fucosyl)idarubicin | - |
? | |
| idarubicin + dTDP-L-2-deoxyfucose | - |
Streptomyces galilaeus ATCC 31615 | dTDP + 4'-O-(alpha-L-fucosyl)idarubicin | - |
? | |
| rhodosaminyl aklavinone + dTDP-L-daunosamine | - |
Streptomyces galilaeus | dTDP + daunosaminyl-rhodosaminyl-aklavinone | - |
? | |
| rhodosaminyl aklavinone + dTDP-L-daunosamine | - |
Streptomyces galilaeus ATCC 31615 | dTDP + daunosaminyl-rhodosaminyl-aklavinone | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50685, calculated from amino acid sequence | Streptomyces galilaeus |
| ? | x * 50699, MALDI-TOF mass spectrometry | Streptomyces galilaeus |
| ? | x * 65000, His6-tagged enzyme, SDS-PAGE | Streptomyces galilaeus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AknK | - |
Streptomyces galilaeus |
| L-2-deoxyfucosyltransferase | - |
Streptomyces galilaeus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
rhodosaminyl aklavinone | with TDP-L-daunosamine as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C | Streptomyces galilaeus | |
| 0.09 | - |
idarubicin | in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C | Streptomyces galilaeus | |
| 1.09 | - |
rhodosaminyl aklavinone | with TDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C | Streptomyces galilaeus | |
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Release 2023.1 (January 2023)
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