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Literature summary for 2.4.1.326 extracted from

  • Leimkuhler, C.; Fridman, M.; Lupoli, T.; Walker, S.; Walsh, C.T.; Kahne, D.
    Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A (2007), J. Am. Chem. Soc., 129, 10546-10550.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
AKnT protein the optimal ratio of AknS to AknT is 1:3 Streptomyces galilaeus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
aklavinone in the presence of activator protein AknT, at pH 7.5 and 25°C Streptomyces galilaeus
0.013
-
aklavinone in the absence of activator protein AknT, at pH 7.5 and 25°C Streptomyces galilaeus
0.28
-
dTDP-beta-L-rhodosamine in the presence of activator protein AknT, at pH 7.5 and 25°C Streptomyces galilaeus
0.349
-
dTDP-beta-L-rhodosamine in the absence of activator protein AknT, at pH 7.5 and 25°C Streptomyces galilaeus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-beta-L-rhodosamine + aklavinone Streptomyces galilaeus
-
dTDP + rhodosaminyl-aklavinone
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces galilaeus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-beta-L-rhodosamine + aklavinone
-
Streptomyces galilaeus dTDP + aclacinomycin T
-
?
dTDP-beta-L-rhodosamine + aklavinone
-
Streptomyces galilaeus dTDP + rhodosaminyl-aklavinone
-
?
dTDP-L-2-deoxyfucose + aklavinone
-
Streptomyces galilaeus dTDP + 4-O-(2-deoxy-alpha-L-fucosyl)aklavinone
-
?
dTDP-L-daunosamine + aklavinone
-
Streptomyces galilaeus dTDP + ?
-
?

Synonyms

Synonyms Comment Organism
AknS/AknT
-
Streptomyces galilaeus
Gtf
-
Streptomyces galilaeus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0083
-
dTDP-beta-L-rhodosamine in the absence of activator protein AknT, at pH 7.5 and 25°C Streptomyces galilaeus
0.16
-
dTDP-beta-L-rhodosamine at pH 7.5 and 25°C Streptomyces galilaeus