Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AKnT protein | the enzyme AknS has very low activity in the absence of the adjacently encoded AknT activator protein. At a 3:1 ratio in the 0.0025-0.01 mM range, AknT stimulates AknS kcat by 40fold for transfer of L-2-deoxyfucose to aklavinone | Streptomyces galilaeus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces galilaeus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
aklavinone | with dTDP-4-amino-2-deoxyrhamnose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
0.0035 | - |
aklavinone | with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
0.046 | - |
epsilon-rhodomycinone | with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
0.156 | - |
dTDP-L-2-deoxyfucose | with epsilon-rhodomycinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
0.2878 | - |
dTDP-L-2-deoxyfucose | with aklavinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
0.4072 | - |
dTDP-4-amino-2-deoxyrhamnose | in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-beta-L-rhodosamine + aklavinone | Streptomyces galilaeus | - |
dTDP + aclacinomycin T | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces galilaeus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography, and gel filtration | Streptomyces galilaeus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-amino-2-deoxyrhamnose + aklavinone | - |
Streptomyces galilaeus | dTDP + 4-O-(2,4-dideoxy-4-amino-alpha-L-rhamnopyranosyl)aklavinone | - |
? | |
dTDP-4-amino-2-deoxyrhamnose + aklavinone | - |
Streptomyces galilaeus | dTDP + 4-O-(2,4-dideoxy-4-amino-alpha-L-rhamnosyl)aklavinone | - |
? | |
dTDP-beta-L-rhodosamine + aklavinone | - |
Streptomyces galilaeus | dTDP + aclacinomycin T | - |
? | |
dTDP-L-2-deoxyfucose + aklavinone | - |
Streptomyces galilaeus | dTDP + 4-O-(2-deoxy-alpha-L-fucosyl)aklavinone | - |
? | |
dTDP-L-2-deoxyfucose + epsilon-rhodomycinone | - |
Streptomyces galilaeus | dTDP + 4-O-(2-deoxy-alpha-L-fucosyl)-epsilon-rhodomycinone | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AknS | - |
Streptomyces galilaeus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
epsilon-rhodomycinone | with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
0.001 | - |
dTDP-L-2-deoxyfucose | with epsilon-rhodomycinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
0.003 | - |
dTDP-4-amino-2-deoxyrhamnose | in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
0.003 | - |
aklavinone | with dTDP-4-amino-2-deoxyrhamnose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
0.004 | - |
dTDP-L-2-deoxyfucose | with aklavinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
0.004 | - |
aklavinone | with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus |