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Literature summary for 2.4.1.32 extracted from

  • Elbein, A.D.
    Biosynthesis of a cell wall glucomannan in mung bean seedlings (1969), J. Biol. Chem., 244, 1608-1616.
    View publication on PubMed
Search for more literature for 2.4.1.32

Inhibitors

Inhibitors Comment Organism Structure
GDP-D-glucose competitive Vigna radiata var. radiata
phosphate buffer slight Vigna radiata var. radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 GDP-D-mannose
-
 Vigna radiata var. radiata

Localization

Localization Comment Organism GeneOntology No. Textmining
particle-bound
-
 Vigna radiata var. radiata
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, optimum concentration: 10 mM Vigna radiata var. radiata

Organism

Organism UniProt Comment Textmining
Vigna radiata var. radiata
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Vigna radiata var. radiata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-D-mannose + (glucomannan)n alkali insoluble glucomannan Vigna radiata var. radiata GDP + (glucomannan)n+1 mannose attached by beta-1,4-linkages ?
GDP-D-mannose + (glucomannan)n specific for GDP-D-mannose as mannosyl donor Vigna radiata var. radiata GDP + (glucomannan)n+1 mannose attached by beta-1,4-linkages ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Vigna radiata var. radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 Tris buffer Vigna radiata var. radiata

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 pH 6.5: 55% of activity maximum, pH 9.0: 10% of activity maximum Vigna radiata var. radiata