Cloned (Comment) | Organism |
---|---|
transient expression as GFP-tagged protein in onion epidermal cells using the CaMV 35S promoter | Delphinium grandiflorum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0229 | - |
cyanidin 3-O-beta-D-glucoside | pH 5.0, 30°C, recombinant enzyme | Delphinium grandiflorum | |
0.2608 | - |
1-O-vanilloyl-beta-D-glucose | pH 5.0, 30°C, recombinant enzyme | Delphinium grandiflorum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vacuole | - |
Delphinium grandiflorum | 5773 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-vanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside | Delphinium grandiflorum | - |
vanillate + cyanidin 3,7-di-O-beta-D-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Delphinium grandiflorum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the enzyme expression is restricted to petals, no activity in leaves and stems, overview | Delphinium grandiflorum | - |
petal | - |
Delphinium grandiflorum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-4-coumaroyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside | - |
Delphinium grandiflorum | 4-coumarate + cyanidin 3,7-di-O-beta-D-glucoside | - |
? | |
1-O-4-hydroxybenzoyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside | 1-O-4-hydroxybenzoyl-beta-D-glucose is the best donor substrate | Delphinium grandiflorum | 4-hydroxybenzoate + cyanidin 3,7-di-O-beta-D-glucoside | - |
? | |
1-O-caffeoyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside | - |
Delphinium grandiflorum | caffeate + cyanidin 3,7-di-O-beta-D-glucoside | - |
? | |
1-O-feruloyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside | - |
Delphinium grandiflorum | ferulate + cyanidin 3,7-di-O-beta-D-glucoside | - |
? | |
1-O-isovanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside | - |
Delphinium grandiflorum | isovanillate + cyanidin 3,7-di-O-beta-D-glucoside | - |
? | |
1-O-vanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-(6-O-malylglucoside) | - |
Delphinium grandiflorum | vanillate + cyanidin 3-O-beta-((6-O-malyl)-D-glucosyl)-7-O-beta-D-glucoside | - |
? | |
1-O-vanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-galactoside | - |
Delphinium grandiflorum | vanillate + cyanidin 3,7-di-O-beta-D-galactoside | - |
? | |
1-O-vanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside | - |
Delphinium grandiflorum | vanillate + cyanidin 3,7-di-O-beta-D-glucoside | - |
? | |
1-O-vanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside | no activity with 1-O-vanilloyl-alpha-D-glucose | Delphinium grandiflorum | vanillate + cyanidin 3,7-di-O-beta-D-glucoside | - |
? | |
1-O-vanilloyl-beta-D-glucose + delphinidin 3-O-beta-D-glucoside | - |
Delphinium grandiflorum | vanillate + delphinidin 3,7-di-O-beta-D-glucoside | - |
? | |
1-O-vanilloyl-beta-D-glucose + pelargonidin 3-O-(6-O-malyl)-beta-D-glucoside | - |
Delphinium grandiflorum | vanillate + pelargonidin 3-O-(6-O-malyl)-beta-D-glucosyl-7-O-beta-D-glucoside | - |
? | |
1-O-vanilloyl-beta-D-glucose + pelargonidin 3-O-beta-D-glucoside | - |
Delphinium grandiflorum | vanillate + pelargonidin 3,7-di-O-beta-D-glucoside | - |
? | |
additional information | substrate specificity, overview. AA7GT uses various acyl-glucose molecules, including benzoyl-glucose derivatives (C6-C1-glucoses) and 1-O-beta-hydroxycinnamoyl-glucoses (HCAGs, C6-C3-glucoses) but not flavonoid glucosides (C6-C3-C6-glucoses), as donor substrates, but it shows a strict acceptor preference for anthocyanidin 3-glucoside, 3-galactoside, and 3-malylglucoside and shows no preferences for other flavonoids or phenolic compounds. No activity with 1-O-beta-D-vanillyl-galactose, 4-nitropheny-glucoside, arbutin, salicin, populin, eleutheroside B, phloridzin, glucovanillin, esculin, kaempferol 3-glucoside, kaempferol 7-neohesperidoside, apigenin 7-glucoside, naringenin 7-glucoside, daidzin, indican. And no activity with kaempferol, kaempferol 3-glucoside, apigenin, apigenin 7-glucoside, naringenin, naringenin 7-glucoside, cyanidin, cyanidin 3,5-glucoside, cyanidin 3,7-glucosside, pelargonidin, delphinidin, (+) catechin, (-) epicatechin, 1-naphtol, 2-naphtol, NAA, indole acetic acid, 7-hydroxy-4-methylcoumarin, 7-hydroxy-6-methoxycoumarin, salicylic acid, phloroglucin | Delphinium grandiflorum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AA7GT | - |
Delphinium grandiflorum |
acyl-glucose-dependent anthocyanin 7-O-glucosyltransferase | - |
Delphinium grandiflorum |
acyl-glucose-dependent glucosyltransferase | - |
Delphinium grandiflorum |
Dg AA7GT | - |
Delphinium grandiflorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Delphinium grandiflorum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
1-O-vanilloyl-beta-D-glucose | pH 5.0, 30°C, recombinant enzyme | Delphinium grandiflorum | |
0.17 | - |
cyanidin 3-O-beta-D-glucoside | pH 5.0, 30°C, recombinant enzyme | Delphinium grandiflorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Delphinium grandiflorum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycoside hydrolase family 1, molecular phylogenetic tree based on amino acid sequences, overview | Delphinium grandiflorum |
physiological function | in delphinium petals, an acyl-glucose, 1-O-beta-D-vanillyl-glucose, acts as the donor molecule in the glucosyl transfer reaction at the 7 position of anthocyanin | Delphinium grandiflorum |