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Literature summary for 2.4.1.300 extracted from

  • Matsuba, Y.; Sasaki, N.; Tera, M.; Okamura, M.; Abe, Y.; Okamoto, E.; Nakamura, H.; Funabashi, H.; Takatsu, M.; Saito, M.; Matsuoka, H.; Nagasawa, K.; Ozeki, Y.
    A novel glucosylation reaction on anthocyanins catalyzed by acyl-glucose-dependent glucosyltransferase in the petals of carnation and delphinium (2010), Plant Cell, 22, 3374-3389.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
transient expression as GFP-tagged protein in onion epidermal cells using the CaMV 35S promoter Delphinium grandiflorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0229
-
cyanidin 3-O-beta-D-glucoside pH 5.0, 30°C, recombinant enzyme Delphinium grandiflorum
0.2608
-
1-O-vanilloyl-beta-D-glucose pH 5.0, 30°C, recombinant enzyme Delphinium grandiflorum

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuole
-
Delphinium grandiflorum 5773
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-O-vanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside Delphinium grandiflorum
-
vanillate + cyanidin 3,7-di-O-beta-D-glucoside
-
?

Organism

Organism UniProt Comment Textmining
Delphinium grandiflorum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information the enzyme expression is restricted to petals, no activity in leaves and stems, overview Delphinium grandiflorum
-
petal
-
Delphinium grandiflorum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-O-4-coumaroyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside
-
Delphinium grandiflorum 4-coumarate + cyanidin 3,7-di-O-beta-D-glucoside
-
?
1-O-4-hydroxybenzoyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside 1-O-4-hydroxybenzoyl-beta-D-glucose is the best donor substrate Delphinium grandiflorum 4-hydroxybenzoate + cyanidin 3,7-di-O-beta-D-glucoside
-
?
1-O-caffeoyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside
-
Delphinium grandiflorum caffeate + cyanidin 3,7-di-O-beta-D-glucoside
-
?
1-O-feruloyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside
-
Delphinium grandiflorum ferulate + cyanidin 3,7-di-O-beta-D-glucoside
-
?
1-O-isovanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside
-
Delphinium grandiflorum isovanillate + cyanidin 3,7-di-O-beta-D-glucoside
-
?
1-O-vanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-(6-O-malylglucoside)
-
Delphinium grandiflorum vanillate + cyanidin 3-O-beta-((6-O-malyl)-D-glucosyl)-7-O-beta-D-glucoside
-
?
1-O-vanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-galactoside
-
Delphinium grandiflorum vanillate + cyanidin 3,7-di-O-beta-D-galactoside
-
?
1-O-vanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside
-
Delphinium grandiflorum vanillate + cyanidin 3,7-di-O-beta-D-glucoside
-
?
1-O-vanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside no activity with 1-O-vanilloyl-alpha-D-glucose Delphinium grandiflorum vanillate + cyanidin 3,7-di-O-beta-D-glucoside
-
?
1-O-vanilloyl-beta-D-glucose + delphinidin 3-O-beta-D-glucoside
-
Delphinium grandiflorum vanillate + delphinidin 3,7-di-O-beta-D-glucoside
-
?
1-O-vanilloyl-beta-D-glucose + pelargonidin 3-O-(6-O-malyl)-beta-D-glucoside
-
Delphinium grandiflorum vanillate + pelargonidin 3-O-(6-O-malyl)-beta-D-glucosyl-7-O-beta-D-glucoside
-
?
1-O-vanilloyl-beta-D-glucose + pelargonidin 3-O-beta-D-glucoside
-
Delphinium grandiflorum vanillate + pelargonidin 3,7-di-O-beta-D-glucoside
-
?
additional information substrate specificity, overview. AA7GT uses various acyl-glucose molecules, including benzoyl-glucose derivatives (C6-C1-glucoses) and 1-O-beta-hydroxycinnamoyl-glucoses (HCAGs, C6-C3-glucoses) but not flavonoid glucosides (C6-C3-C6-glucoses), as donor substrates, but it shows a strict acceptor preference for anthocyanidin 3-glucoside, 3-galactoside, and 3-malylglucoside and shows no preferences for other flavonoids or phenolic compounds. No activity with 1-O-beta-D-vanillyl-galactose, 4-nitropheny-glucoside, arbutin, salicin, populin, eleutheroside B, phloridzin, glucovanillin, esculin, kaempferol 3-glucoside, kaempferol 7-neohesperidoside, apigenin 7-glucoside, naringenin 7-glucoside, daidzin, indican. And no activity with kaempferol, kaempferol 3-glucoside, apigenin, apigenin 7-glucoside, naringenin, naringenin 7-glucoside, cyanidin, cyanidin 3,5-glucoside, cyanidin 3,7-glucosside, pelargonidin, delphinidin, (+) catechin, (-) epicatechin, 1-naphtol, 2-naphtol, NAA, indole acetic acid, 7-hydroxy-4-methylcoumarin, 7-hydroxy-6-methoxycoumarin, salicylic acid, phloroglucin Delphinium grandiflorum ?
-
?

Synonyms

Synonyms Comment Organism
AA7GT
-
Delphinium grandiflorum
acyl-glucose-dependent anthocyanin 7-O-glucosyltransferase
-
Delphinium grandiflorum
acyl-glucose-dependent glucosyltransferase
-
Delphinium grandiflorum
Dg AA7GT
-
Delphinium grandiflorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Delphinium grandiflorum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
1-O-vanilloyl-beta-D-glucose pH 5.0, 30°C, recombinant enzyme Delphinium grandiflorum
0.17
-
cyanidin 3-O-beta-D-glucoside pH 5.0, 30°C, recombinant enzyme Delphinium grandiflorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Delphinium grandiflorum

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycoside hydrolase family 1, molecular phylogenetic tree based on amino acid sequences, overview Delphinium grandiflorum
physiological function in delphinium petals, an acyl-glucose, 1-O-beta-D-vanillyl-glucose, acts as the donor molecule in the glucosyl transfer reaction at the 7 position of anthocyanin Delphinium grandiflorum