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Literature summary for 2.4.1.297 extracted from

  • Arita, T.; Miyazaki, S.; Teramoto, S.; Yoshitama, K.
    Major anthocyanin biosynthesis in the brilliant crimson petals from Erythrina crista-galli L (2014), Sci. Hortic., 168, 272-280.
No PubMed abstract available
Search for more literature for 2.4.1.297

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 1 mM, 125% of initial activity Erythrina crista-galli

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 84% residual activity Erythrina crista-galli
4-chloromercuribenzoate 1 mM, 4% residual activity Erythrina crista-galli
Co2+ 1 mM, 31% residual activity Erythrina crista-galli
Cu2+ 1 mM, 10% residual activity Erythrina crista-galli
additional information not inhibitory: EDTA at 1 mM Erythrina crista-galli
N-ethylmaleimide 1 mM, 16.4% residual activity Erythrina crista-galli
Zn2+ 1 mM, 3.5% residual activity Erythrina crista-galli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 gel fitlration Erythrina crista-galli

Organism

Organism UniProt Comment Textmining
Erythrina crista-galli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from petal Erythrina crista-galli

Source Tissue

Source Tissue Comment Organism Textmining
petal
-
 Erythrina crista-galli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme catalyzes only the 2''-O-glucosylation of flavonoid 3-O-glucosideto produce flavonoid 3-O-sophoroside. Both anthocyanidin 3-O-glucosides and flavonol 3-O-glucosides are favorable acceptor substrates of the enzyme, whereas no activity is shown toward C-glucosylflavones such as isovitexin Erythrina crista-galli ?
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-galactoside 9.1% of the activity with cyanidin 3-O-beta-D-glucoside Erythrina crista-galli UDP + cyanidin 2''-O-alpha-D-glucoside 3-O-beta-D-galactoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
 Erythrina crista-galli UDP + cyanidin 3-O-sophoroside
-
 ?
UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside 64.8% of the activity with cyanidin 3-O-beta-D-glucoside Erythrina crista-galli UDP + delphinidin 3-O-sophoroside
-
 ?
UDP-alpha-D-glucose + kaempferol 3-O-beta-D-glucoside 41.6% of the activity with cyanidin 3-O-beta-D-glucoside Erythrina crista-galli UDP + kaempferol 3-O-sophoroside
-
 ?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside 29.3% of the activity with cyanidin 3-O-beta-D-glucoside Erythrina crista-galli UDP + pelargonidin 3-O-sophoroside
-
 ?
UDP-alpha-D-glucose + quercetin 3-O-beta-D-glucoside 33.8% of the activity with cyanidin 3-O-beta-D-glucoside Erythrina crista-galli UDP + quercetin 3-O-sophoroside
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 82000, SDS-PAGE Erythrina crista-galli

Synonyms

Synonyms Comment Organism
3GGT
-
 Erythrina crista-galli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Erythrina crista-galli

pI Value

Organism Comment pI Value Maximum pI Value
Erythrina crista-galli isoelectric focusing
-
 6.5