Cloned (Comment) | Organism |
---|---|
gene pglH, overexpression of His-tagged PglH in Escherichia coli | Campylobacter jejuni |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | asociated | Campylobacter jejuni | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
x * 41000, recombinant His-tagged PglH, SDS-PAGE | Campylobacter jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | Campylobacter jejuni | - |
3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
gene pglH, pgl gene cluster | - |
Campylobacter jejuni NCTC 11168 | - |
gene pglH, pgl gene cluster | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged PglH from Escherichia coli by nickel affinity chromatography | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | - |
Campylobacter jejuni | 3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
additional information | in vitro, trisaccharide is prepared by using PglA and PglJ and then reacts singly with PglH | Campylobacter jejuni | ? | - |
? | |
additional information | in vitro, trisaccharide is prepared by using PglA and PglJ and then reacts singly with PglH | Campylobacter jejuni NCTC 11168 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41000, recombinant His-tagged PglH, SDS-PAGE | Campylobacter jejuni |
Synonyms | Comment | Organism |
---|---|---|
PglH | - |
Campylobacter jejuni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | assay at | Campylobacter jejuni |
General Information | Comment | Organism |
---|---|---|
metabolism | PglH is part of a general N-linked glycosylation pathway, encoded by the pgl gene cluster, which culminates in the transfer of a heptasaccharide: GalNAc-alpha1,4-GalNAc-alpha1,4-(Glcbeta1,3)-GalNAc-alpha1,4-GalNAc-alpha1,4-GalNAc-alpha1,3-Bac, where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose), from a membrane-anchored undecaprenylpyrophosphate-linked donor to the asparagine side chain of proteins at the Asn-X-Ser/Thr motif. The glycosyltransferases, PglA, PglH, PglI, and PglJ, responsible for the biosynthesis of the Und-PP-linked heptasaccharide. Elongation of the trisaccharide with PglH results in a hexasaccharide revealing the polymerase activity of PglH | Campylobacter jejuni |