Literature summary for 2.4.1.288 extracted from

Rose, N.L.; Zheng, R.B.; Pearcey, J.; Zhou, R.; Completo, G.C.; Lowary, T.L.
Development of a coupled spectrophotometric assay for GlfT2, a bifunctional mycobacterial galactofuranosyltransferase (2008), Carbohydr. Res., 343, 2130-2139.

Search for more literature for 2.4.1.288

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta (DE3) Quarters cells	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0038	-	UDP-alpha-D-galactofuranose	apparent value, spectrophotometric assay, in 50 mM MOPS, pH 7.9, 50 mM KCl, 20 mM MgCl2, at 37°C	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
73500	-	x * 73500, His-tagged enzyme, estimated from SDS-PAGE	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-NTA agarose column chromatography and Sephacryl S-100 gel filtration	Mycobacterium tuberculosis

Storage Stability

Storage Stability	Organism
-80°C, purified enzyme with 20% (v/v) glycerol, four months, no significant loss of activity	Mycobacterium tuberculosis
4°C, purified enzyme with 20% (v/v) glycerol, 2 weeks, no significant loss of activity	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol	the enzyme adds the third and subsequent alpha-D-galactofuranosyl residues to the growing galactan chain	Mycobacterium tuberculosis	UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol	-	?
UDP-alpha-D-galactofuranose + beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-O(CH2)7CH3	-	Mycobacterium tuberculosis	UDP + ?	-	?
UDP-alpha-D-galactofuranose + beta-D-Galf-(1->6)-beta-D-Galf-(1->5)-beta-D-Galf-O(CH2)7CH3	-	Mycobacterium tuberculosis	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 73500, His-tagged enzyme, estimated from SDS-PAGE	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
galactofuranosyltransferase	-	Mycobacterium tuberculosis
GlfT2	-	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
7.2	-	UDP-alpha-D-galactofuranose	spectrophotometric assay, in 50 mM MOPS, pH 7.9, 50 mM KCl, 20 mM MgCl2, at 37°C	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)