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Literature summary for 2.4.1.288 extracted from

  • May, J.F.; Levengood, M.R.; Splain, R.A.; Brown, C.D.; Kiessling, L.L.
    A processive carbohydrate polymerase that mediates bifunctional catalysis using a single active site (2012), Biochemistry, 51, 1148-1159.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.288

Protein Variants

Protein Variants Comment Organism
D256A the mutant shows almost no activity even in the presence of Mn2+ or Mg2+ Mycobacterium tuberculosis
D256E the mutant shows almost no activity even in the presence of Mn2+ or Mg2+ Mycobacterium tuberculosis
D258A the mutant shows almost no activity in the presence of Mg2+, but wild type activity in the presence of Mn2+ Mycobacterium tuberculosis
D258E the mutant shows almost no activity in the presence of Mg2+, and about 10% activity in the presence of Mn2+ as compared to the wild type enzyme Mycobacterium tuberculosis
D371A inactive Mycobacterium tuberculosis
D372A inactive Mycobacterium tuberculosis
D372E inactive Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ essential for catalysis Mycobacterium tuberculosis
Mn2+ essential for catalysis Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 Mycobacterium tuberculosis UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?
UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 Mycobacterium tuberculosis H37Rv UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?

Synonyms

Synonyms Comment Organism
GlfT2
-
 Mycobacterium tuberculosis
Rv3808c
-
 Mycobacterium tuberculosis

General Information

General Information Comment Organism
metabolism GlfT2 catalyzes the formation of mycobacterial galactan, mediating galactan polymerization using only one active site that manifests dual regioselectivity Mycobacterium tuberculosis