Crystallization (Comment) | Organism |
---|---|
crystal structure with/without 4-O-beta-D-mannosyl-D-glucose. His245 of loop 3 forms a hydrogen-bond network with the substrate through a water molecule, and is indispensible for substrate binding | Ruminococcus albus |
Protein Variants | Comment | Organism |
---|---|---|
H245A | 1.47% of wild-type phosphorolytic activity | Ruminococcus albus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.41 | - |
alpha-D-mannose 1-phosphate | wild-type, pH 6.5, 37°C | Ruminococcus albus | |
25.9 | - |
D-glucose | wild-type, pH 6.5, 37°C | Ruminococcus albus | |
36.5 | - |
alpha-D-mannose 1-phosphate | mutant H245A, pH 6.5, 37°C | Ruminococcus albus | |
319 | - |
D-glucose | mutant H245A, pH 6.5, 37°C | Ruminococcus albus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruminococcus albus | E6UIS7 | - |
- |
Ruminococcus albus DSM 20455 | E6UIS7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate | - |
? | |
D-glucose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus DSM 20455 | 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MP1 | - |
Ruminococcus albus |
Rumal_0852 | - |
Ruminococcus albus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.76 | - |
alpha-D-mannose 1-phosphate | mutant H245A, pH 6.5, 37°C | Ruminococcus albus | |
19.3 | - |
D-glucose | mutant H245A, pH 6.5, 37°C | Ruminococcus albus | |
52.1 | - |
alpha-D-mannose 1-phosphate | wild-type, pH 6.5, 37°C | Ruminococcus albus | |
126 | - |
D-glucose | wild-type, pH 6.5, 37°C | Ruminococcus albus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
D-glucose | mutant H245A, pH 6.5, 37°C | Ruminococcus albus | |
0.076 | - |
alpha-D-mannose 1-phosphate | mutant H245A, pH 6.5, 37°C | Ruminococcus albus | |
4.86 | - |
D-glucose | wild-type, pH 6.5, 37°C | Ruminococcus albus | |
21.6 | - |
alpha-D-mannose 1-phosphate | wild-type, pH 6.5, 37°C | Ruminococcus albus |