Literature summary for 2.4.1.269 extracted from

Nobre, A.; Empadinhas, N.; Nobre, M.; Lourenco, E.; Maycock, C.; Ventura, M.; Mingote, A.; da Costa, M.
The plant Selaginella moellendorffii possesses enzymes for synthesis and hydrolysis of the compatible solutes mannosylglycerate and glucosylglycerate (2012), Planta, 237, 891-901.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Selaginella moellendorffii

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	divalent cation inhibit in decreasing order: Sr2+, Ni2, Co2+, Ca2+, Mn2+, Zn2+	Selaginella moellendorffii
Co2+	divalent cation inhibit in decreasing order: Sr2+, Ni2, Co2+, Ca2+, Mn2+, Zn2+	Selaginella moellendorffii
Mn2+	divalent cation inhibit in decreasing order: Sr2+, Ni2, Co2+, Ca2+, Mn2+, Zn2+	Selaginella moellendorffii
Ni2+	divalent cation inhibit in decreasing order: Sr2+, Ni2, Co2+, Ca2+, Mn2+, Zn2+	Selaginella moellendorffii
Sr2+	divalent cation inhibit in decreasing order: Sr2+, Ni2, Co2+, Ca2+, Mn2+, Zn2+	Selaginella moellendorffii
Zn2+	divalent cation inhibit in decreasing order: Sr2+, Ni2, Co2+, Ca2+, Mn2+, Zn2+	Selaginella moellendorffii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2	-	GDP-mannose	pH 8.0, 25°C	Selaginella moellendorffii
0.6	-	GDP-mannose	pH 8.0, 25°C	Selaginella moellendorffii
1	-	GDP-mannose	pH 8.0, 50°C	Selaginella moellendorffii
1.2	-	D-glycerate	cosubstrate GDP-mannose, pH 8.0, 25°C	Selaginella moellendorffii
1.6	-	D-glycerate	cosubstrate GDP-glucose, pH 8.0, 25°C	Selaginella moellendorffii
2.4	-	GDP-mannose	pH 8.0, 50°C	Selaginella moellendorffii
3.4	-	D-glycerate	cosubstrate GDP-glucose, pH 8.0, 50°C	Selaginella moellendorffii
4.4	-	D-glycerate	cosubstrate GDP-mannose, pH 8.0, 50°C	Selaginella moellendorffii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	maximum activity at 5 mM	Selaginella moellendorffii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47900	-	4 * 47900, calculated	Selaginella moellendorffii
217200	-	gel filtration	Selaginella moellendorffii

Organism

Organism	UniProt	Comment	Textmining
Selaginella moellendorffii	-	-	-

Storage Stability

Storage Stability	Organism
0°C, stable for more than 2 months	Selaginella moellendorffii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GDP-alpha-D-glucose + D-glycerate	-	Selaginella moellendorffii	2-O-(alpha-D-glucopyranosyl)-D-glycerate + GDP	-	?
GDP-mannose + D-glycerate	-	Selaginella moellendorffii	2-O-(alpha-D-mannopyranosyl)-D-glycerate + GDP	-	?
additional information	enzyme is able to efficiently synthesize mannosylglycerate and glucosylglycerate alike	Selaginella moellendorffii	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 47900, calculated	Selaginella moellendorffii

Synonyms

Synonyms	Comment	Organism
MGS	-	Selaginella moellendorffii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Selaginella moellendorffii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Selaginella moellendorffii

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Release 2023.1 (January 2023)