Protein Variants | Comment | Organism |
---|---|---|
D134A | substitution of a canonical GT-A D134XD136 aspartic acid, complete loss of activity | Mycobacterium tuberculosis |
D136A | substitution of a canonical GT-A D134XD136 aspartic acid, complete loss of activity | Mycobacterium tuberculosis |
E232A | kinetic parameters similar to wild-type | Mycobacterium tuberculosis |
H258A | 2540-fold increases in Km values | Mycobacterium tuberculosis |
R256A | mutation in a flexible loop covering the active site, strong increase in Km values | Mycobacterium tuberculosis |
R261A | kinetic parameters similar to wild-type | Mycobacterium tuberculosis |
T187A | 100fold decrease in the value of kcat | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-phospho-D-glycerate | competitive with UDP-glucose | Mycobacterium tuberculosis | |
UMP | competitive with UDP-glucose, uncompetitive with 3-phospho-D-glycerate | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
3-phospho-D-glycerate | pH 7.8, 25°C | Mycobacterium tuberculosis | |
0.25 | - |
3-phospho-D-glycerate | mutant E232A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
0.48 | - |
UDP-glucose | mutant T187A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
0.58 | - |
UDP-glucose | mutant E232A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
0.74 | - |
3-phospho-D-glycerate | mutant T187A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
0.82 | - |
UDP-glucose | wild-type, pH 7.8, 25°C | Mycobacterium tuberculosis | |
1.1 | - |
3-phospho-D-glycerate | mutant R261A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
1.5 | - |
UDP-glucose | mutant R261A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
8 | - |
3-phospho-D-glycerate | mutant H258A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
20 | - |
UDP-glucose | mutant H258A, pH 7.8, 25°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WMW9 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P9WMW9 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
NDP-glucose + 3-phospho-D-glycerate = NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | ordered kinetic mechanism with 3-phosphoglycerate binding first, followed by UDP-glucose | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | a single ionizable residue is involved catalysis (pKa = 6.3) that must be deprotonated for full activity. A solvent kinetic isotope effect of 2.0 on kcat is consistent with a proton in flight during the rate-determining step | Mycobacterium tuberculosis | ? | - |
? | |
additional information | a single ionizable residue is involved catalysis (pKa = 6.3) that must be deprotonated for full activity. A solvent kinetic isotope effect of 2.0 on kcat is consistent with a proton in flight during the rate-determining step | Mycobacterium tuberculosis ATCC 25618 | ? | - |
? | |
UDP-glucose + 3-phospho-D-glycerate | - |
Mycobacterium tuberculosis | UDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
UDP-glucose + 3-phospho-D-glycerate | - |
Mycobacterium tuberculosis ATCC 25618 | UDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
UDP-glucose | mutant T187A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
0.38 | - |
UDP-glucose | mutant R261A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
0.75 | - |
UDP-glucose | mutant H258A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
0.82 | - |
UDP-glucose | wild-type, pH 7.8, 25°C | Mycobacterium tuberculosis | |
0.83 | - |
UDP-glucose | mutant E232A, pH 7.8, 25°C | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
UMP | substrate UDP-glucose, pH 7.8, 25°C | Mycobacterium tuberculosis | |
0.22 | - |
UMP | substrate 3-phospho-D-glycerate, pH 7.8, 25°C | Mycobacterium tuberculosis | |
21 | - |
3-phospho-D-glycerate | substrate UDP-glucose, pH 7.8, 25°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
3-phospho-D-glycerate | mutant T187A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
0.024 | - |
UDP-glucose | mutant T187A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
0.034 | - |
3-phospho-D-glycerate | mutant H258A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
0.038 | - |
UDP-glucose | mutant H258A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
0.248 | - |
UDP-glucose | mutant R261A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
0.31 | - |
3-phospho-D-glycerate | mutant R261A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
1.12 | - |
UDP-glucose | wild-type, pH 7.8, 25°C | Mycobacterium tuberculosis | |
1.28 | - |
UDP-glucose | mutant E232A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
3.42 | - |
3-phospho-D-glycerate | mutant E232A, pH 7.8, 25°C | Mycobacterium tuberculosis | |
3.58 | - |
3-phospho-D-glycerate | pH 7.8, 25°C | Mycobacterium tuberculosis |