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Literature summary for 2.4.1.266 extracted from

  • Cunha, S.; dAvo, A.F.; Mingote, A.; Lamosa, P.; da Costa, M.S.; Costa, J.
    Mannosylglucosylglycerate biosynthesis in the deep-branching phylum Planctomycetes: characterization of the uncommon enzymes from Rhodopirellula baltica (2013), Sci. Rep., 3, 2378.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodopirellula baltica

Inhibitors

Inhibitors Comment Organism Structure
EDTA 2.5 mM, 19% residual activity Rhodopirellula baltica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
3-phospho-D-glycerate pH 7.5, 25°C Rhodopirellula baltica
0.7
-
ADP-glucose pH 7.5, 25°C Rhodopirellula baltica

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ and Mn2+, stimulation Rhodopirellula baltica
Mg2+ maximum activtiy in presence of 1 mM Mg2+ Rhodopirellula baltica
Mn2+ and Co2+, stimulation Rhodopirellula baltica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44990
-
5 * 44990, calculated Rhodopirellula baltica
218600
-
gel filtration Rhodopirellula baltica

Organism

Organism UniProt Comment Textmining
Rhodopirellula baltica Q7UXY8
-
-
Rhodopirellula baltica DSM 10527 Q7UXY8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
substrate GDP-glucose, pH 7.5, 25°C Rhodopirellula baltica
0.17
-
substrate UDP-glucose, pH 7.5, 25°C Rhodopirellula baltica
1.1
-
substrate ADP-glucose, pH 7.5, 25°C Rhodopirellula baltica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + 3-phospho-D-glycerate
-
Rhodopirellula baltica ADP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
ADP-glucose + 3-phospho-D-glycerate
-
Rhodopirellula baltica DSM 10527 ADP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
GDP-glucose + 3-phospho-D-glycerate
-
Rhodopirellula baltica GDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
GDP-glucose + 3-phospho-D-glycerate
-
Rhodopirellula baltica DSM 10527 GDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
additional information enzyme is rather non-specific for glucosyl donors using several glucose diphosphate nucleosides, with ADP-glucose being by far the preferred substrate, followed by UDP-glucose and GDP-glucose. Only 3-PGA can used as glucosyl acceptor leading to the formation of glucosyl 3-phosphoglycerate Rhodopirellula baltica ?
-
?
additional information enzyme is rather non-specific for glucosyl donors using several glucose diphosphate nucleosides, with ADP-glucose being by far the preferred substrate, followed by UDP-glucose and GDP-glucose. Only 3-PGA can used as glucosyl acceptor leading to the formation of glucosyl 3-phosphoglycerate Rhodopirellula baltica DSM 10527 ?
-
?
UDP-glucose + 3-phospho-D-glycerate
-
Rhodopirellula baltica UDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
UDP-glucose + 3-phospho-D-glycerate
-
Rhodopirellula baltica DSM 10527 UDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?

Subunits

Subunits Comment Organism
pentamer 5 * 44990, calculated Rhodopirellula baltica

Synonyms

Synonyms Comment Organism
GpgS
-
Rhodopirellula baltica
RB1005
-
Rhodopirellula baltica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Rhodopirellula baltica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Rhodopirellula baltica

pI Value

Organism Comment pI Value Maximum pI Value
Rhodopirellula baltica calculated
-
5.4