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Literature summary for 2.4.1.266 extracted from
- Single-step pathway for synthesis of glucosylglycerate in Persephonella marina (2007), J. Bacteriol., 189, 4014-4019.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gpgS, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3), the genes for glucosyl-3-phosphoglycerate synthase, GpgS, and glucosyl-3-phosphoglycerate phosphatase, GpgP, the enzymes that lead to the synthesis of glucosylglycerol through the formation of glucosyl-3-phosphoglycerate, are organized in anoperon-like structure with the gene encoding a putative glycosyltransferase, the glucosylglycerate synthase, Ggs, overview | Persephonella marina |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, recombinant enzyme | Persephonella marina |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Persephonella marina |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Persephonella marina | the genes for glucosyl-3-phosphoglycerate synthase, GpgS, and glucosyl-3-phosphoglycerate phosphatase, GpgP, the enzymes that lead to the synthesis of glucosylglycerol through the formation of glucosyl-3-phosphoglycerate, are organized in anoperon-like structure with the gene encoding a putative glycosyltransferase, the glucosylglycerate synthase, Ggs, overview, biosynthetic pathway overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Persephonella marina | A9QXB4 | strain DSM 14350 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-alpha-D-glucose + D-3-phosphoglycerate | - |
Persephonella marina | ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
| GDP-glucose + D-3-phosphoglycerate | - |
Persephonella marina | GDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate | - |
? | |
| additional information | the genes for glucosyl-3-phosphoglycerate synthase, GpgS, and glucosyl-3-phosphoglycerate phosphatase, GpgP, the enzymes that lead to the synthesis of glucosylglycerol through the formation of glucosyl-3-phosphoglycerate, are organized in anoperon-like structure with the gene encoding a putative glycosyltransferase, the glucosylglycerate synthase, Ggs, overview, biosynthetic pathway overview | Persephonella marina | ? | - |
? | |
| UDP-glucose + D-3-phosphoglycerate | - |
Persephonella marina | UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucosyl-3-phosphoglycerate synthase | - |
Persephonella marina |
| GpgS | - |
Persephonella marina |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
assay at | Persephonella marina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Persephonella marina |
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Release 2023.1 (January 2023)
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