KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0085 | - |
UDP-GlcNAc | pH 7.4, 37°C | Homo sapiens | |
0.1418 | - |
UDP-6-deoxy-GlcNAc | pH 7.4, 37°C | Homo sapiens | |
0.2821 | - |
UDP-GlcNPr | pH 7.4, 37°C | Homo sapiens | |
0.3695 | - |
UDP-4-deoxy-GalNAc | pH 7.4, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O15294 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
KKKYPGGSTPVSSANMM + UDP-4-deoxy-GalNAc | - |
Homo sapiens | ? + UDP | - |
? | |
KKKYPGGSTPVSSANMM + UDP-4-deoxy-GlcNAc | 22.2% yield | Homo sapiens | ? + UDP | - |
? | |
KKKYPGGSTPVSSANMM + UDP-6-deoxy-GalNAc | 37.7% yield | Homo sapiens | ? + UDP | - |
? | |
KKKYPGGSTPVSSANMM + UDP-6-deoxy-GlcNAc | 85% yield | Homo sapiens | ? + UDP | - |
? | |
KKKYPGGSTPVSSANMM + UDP-GlcNAc | peptide acceptor derived from casein kinase II | Homo sapiens | ? + UDP | - |
? | |
KKKYPGGSTPVSSANMM + UDP-GlcNAz | peptide acceptor derived from casein kinase II | Homo sapiens | ? + UDP | - |
? | |
KKKYPGGSTPVSSANMM + UDP-GlcNPr | the close vicinity between Met501 and the N-acyl group of GlcNPr, as well as the hydrophobic environment near Met501, are responsible for the selective binding of UDP-GlcNPr | Homo sapiens | ? + UDP | - |
? | |
additional information | the substitution of the N-acyl group, deoxy modification of C6/C4-OH or epimerization of C4-OH of the GlcNAc in UDP-GlcNAc decrease its affinity to isoform short OGT. The backbone carbonyl oxygen of Leu653 and the hydroxyl group of Thr560 in sOGT contribute to the recognition of the sugar moiety via hydrogen bonds | Homo sapiens | ? | - |
? |