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Literature summary for 2.4.1.255 extracted from

  • Jiang, J.; Lazarus, M.B.; Pasquina, L.; Sliz, P.; Walker, S.
    A neutral diphosphate mimic crosslinks the active site of human O-GlcNAc transferase (2012), Nat. Chem. Biol., 8, 72-77.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Homo sapiens

Protein Variants

Protein Variants Comment Organism
C835A the mutation has no effect compared with wild type enzyme Homo sapiens
C911A active site mutant Homo sapiens
C917A active site mutant Homo sapiens
K842A active site mutant Homo sapiens
T914A active site mutant Homo sapiens
T921A active site mutant Homo sapiens
T922A active site mutant Homo sapiens
Y841A the mutation has no effect compared with wild type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-methoxyphenyl 5-acetyl-3-hydroxy-2-oxo-2,3-dihydro-1H-indole-1-carboxylate the compound fully inactivates the enzyme within 5 min at a 1:1 ratio of inhibitor:enzyme Homo sapiens
4-methoxyphenyl 6-chloro-3-hydroxy-2-oxo-2,3-dihydro-1H-indole-1-carboxylate about 30% inhibition with a 3fold excess of inhibitor Homo sapiens
phenyl 3-hydroxy-2-oxo-2,3-dihydro-1H-indole-1-carboxylate about 70% inhibition with a 3fold excess of inhibitor Homo sapiens
phenyl 3-hydroxy-5-methoxy-2-oxo-2,3-dihydro-1H-indole-1-carboxylate about 10% inhibition with a 3fold excess of inhibitor Homo sapiens
phenyl 3-hydroxy-5-nitro-2-oxo-2,3-dihydro-1H-indole-1-carboxylate about 60% inhibition with a 3fold excess of inhibitor Homo sapiens
phenyl 6-chloro-3-hydroxy-2-oxo-2,3-dihydro-1H-indole-1-carboxylate the compound causes an irreversible loss of enzyme activity, about 48% inhibition with a 3fold excess of inhibitor Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O15294
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + KKKYPGGSTPVSSANMM
-
Homo sapiens UDP + ?
-
?

Synonyms

Synonyms Comment Organism
O-GlcNAc transferase
-
Homo sapiens
O-linked N-acetylglucosamine transferase
-
Homo sapiens
OGT
-
Homo sapiens