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Literature summary for 2.4.1.255 extracted from

  • Okuyama, R.; Marshall, S.
    UDP-N-acetylglucosaminyl transferase (OGT) in brain tissue: temperature sensitivity and subcellular distribution of cytosolic and nuclear enzyme (2003), J. Neurochem., 86, 1271-1280.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ATP has a much lower affinity for OGT Rattus norvegicus
KCl lowers OGT activity by reducing enzyme affinity for UDP-GlcNAc Rattus norvegicus
NaCl
-
Rattus norvegicus
NaH2PO4
-
Rattus norvegicus
UDP-galactose much lower affinity for OGT compared with UDP-GlcNAc Rattus norvegicus
UDP-GlcNAc
-
Rattus norvegicus
UDP-glucose much lower affinity for OGT compared with UDP-GlcNAc Rattus norvegicus
UTP has a high affinity for OGT Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-
nucleus
-
Rattus norvegicus 5634
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immunopurified Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
adipose
-
Rattus norvegicus
-
brain cytosolic OGT activity is 10times more abundant in brain tissue compared with muscle, adipose, heart, and liver tissue Rattus norvegicus
-
heart
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
muscle
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-GlcNAc + beta-amyloid associated protein
-
Rattus norvegicus GlcNAc-beta-amyloid associated protein + UDP
-
?
UDP-GlcNAc + tau protein
-
Rattus norvegicus GlcNAc-tau protein + UDP
-
?

Synonyms

Synonyms Comment Organism
O-GlcNAc transferase
-
Rattus norvegicus
OGT
-
Rattus norvegicus
UDP-N-acetylglucosaminyl transferase
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24 27 a slight loss in enzyme activity is seen at 30°C, almost a complete loss of activity occurs at 37°C. At 33°C there is a 45% decrease in OGT activity Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
cytosolic OGT activity is stable at 24°C but was rapidly inactivated at 37°C (t1/2 = 20 min). Nuclear OGT is not inactivated at 37°C Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00025
-
-
Rattus norvegicus UDP-GlcNAc
0.0005
-
-
Rattus norvegicus UTP
0.0042
-
much lower affinity for OGT compared with UDP-GlcNAc Rattus norvegicus UDP-glucose
0.013
-
much lower affinity for OGT compared with UDP-GlcNAc Rattus norvegicus UDP-galactose
0.86
-
-
Rattus norvegicus ATP
4
-
about Rattus norvegicus NaCl
45
-
-
Rattus norvegicus NaH2PO4
50
-
about Rattus norvegicus KCl