Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | has a much lower affinity for OGT | Rattus norvegicus | |
KCl | lowers OGT activity by reducing enzyme affinity for UDP-GlcNAc | Rattus norvegicus | |
NaCl | - |
Rattus norvegicus | |
NaH2PO4 | - |
Rattus norvegicus | |
UDP-galactose | much lower affinity for OGT compared with UDP-GlcNAc | Rattus norvegicus | |
UDP-GlcNAc | - |
Rattus norvegicus | |
UDP-glucose | much lower affinity for OGT compared with UDP-GlcNAc | Rattus norvegicus | |
UTP | has a high affinity for OGT | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
nucleus | - |
Rattus norvegicus | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immunopurified | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipose | - |
Rattus norvegicus | - |
brain | cytosolic OGT activity is 10times more abundant in brain tissue compared with muscle, adipose, heart, and liver tissue | Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
muscle | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-GlcNAc + beta-amyloid associated protein | - |
Rattus norvegicus | GlcNAc-beta-amyloid associated protein + UDP | - |
? | |
UDP-GlcNAc + tau protein | - |
Rattus norvegicus | GlcNAc-tau protein + UDP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
O-GlcNAc transferase | - |
Rattus norvegicus |
OGT | - |
Rattus norvegicus |
UDP-N-acetylglucosaminyl transferase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | 27 | a slight loss in enzyme activity is seen at 30°C, almost a complete loss of activity occurs at 37°C. At 33°C there is a 45% decrease in OGT activity | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
cytosolic OGT activity is stable at 24°C but was rapidly inactivated at 37°C (t1/2 = 20 min). Nuclear OGT is not inactivated at 37°C | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00025 | - |
- |
Rattus norvegicus | UDP-GlcNAc | |
0.0005 | - |
- |
Rattus norvegicus | UTP | |
0.0042 | - |
much lower affinity for OGT compared with UDP-GlcNAc | Rattus norvegicus | UDP-glucose | |
0.013 | - |
much lower affinity for OGT compared with UDP-GlcNAc | Rattus norvegicus | UDP-galactose | |
0.86 | - |
- |
Rattus norvegicus | ATP | |
4 | - |
about | Rattus norvegicus | NaCl | |
45 | - |
- |
Rattus norvegicus | NaH2PO4 | |
50 | - |
about | Rattus norvegicus | KCl |