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Literature summary for 2.4.1.255 extracted from

  • Lubas, W.A.; Hanover, J.A.
    Functional expression of O-linked GlcNAc transferase: domain structure and substrate specificity (2000), J. Biol. Chem., 275, 10983-10988.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information deletion mutants of OGT variant A-G. Deletions in the highly conserved C-terminus result in a complete loss of activity. The N-terminal tetratricopeptide repeat domain is required for optimal recognition of substrates. Removal of the first three tetratricopeptide repeats greatly reduces the O-GlcNAc addition to macromolecular substrates Homo sapiens
additional information deletion of the first 3 tetratricopeptide repeats in clone B results in a significant loss of activity (58%) for Nup 62 Homo sapiens
additional information removal of the first 6 tetratricopeptide repeats in clone C results in an almost complete loss of activity toward Nup 62 as a substrate Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
UDP completely inhibiting Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
UDP-GlcNAc
-
Homo sapiens
0.0012
-
Nup 62 protein
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
103000
-
-
Homo sapiens
114000
-
fusion protein Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme modifies nuclear pore proteins and transcription factors ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
clone Lv4F
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein kinase II peptide + UDP-GlcNAc much poorer substrate than Nup 62 Homo sapiens UDP + ?
-
?
GSK-3beta + UDP-GlcNAc rabbit skeletal muscle glycogen synthase kinase (GSK) -3beta Homo sapiens UDP + ?
-
?
additional information enzyme modifies nuclear pore proteins and transcription factors Homo sapiens ?
-
?
additional information enzyme does not modify peptide YSDSGSTST, cAMP-dependent protein kinase A, casein kinase I, mitogen activated protein kinase (extracellular signal-regulated kinase 2), or calmodulin-dependent protein kinase II Homo sapiens ?
-
?
UDP-GlcNAc + Nup 62 protein
-
Homo sapiens UDP + ?
-
?
YSDSPSTST + UDP-GlcNAc
-
Homo sapiens UDP + ?
-
?

Synonyms

Synonyms Comment Organism
human OGT
-
Homo sapiens
O-linked GlcNAc transferase
-
Homo sapiens
OGT
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens