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Literature summary for 2.4.1.251 extracted from

  • Salinas, S.R.; Bianco, M.I.; Barreras, M.; Ielpi, L.
    Expression, purification and biochemical characterization of GumI, a monotopic membrane GDP-mannose:glycolipid 4-beta-D-mannosyltransferase from Xanthomonas campestris pv. campestris (2011), Glycobiology, 21, 903-913.
    View publication on PubMed
Search for more literature for 2.4.1.251

Activating Compound

Activating Compound Comment Organism Structure
dodecylmaltoside maximal activity in the presence of dodecylmaltoside at a concentration of 33.3 critical micelle concentration Xanthomonas campestris
Triton X-100 maximal activity at 133 critical micelle concentration or 32 mM or 2%, representing about 45% of that observed with dodecylmaltoside Xanthomonas campestris

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Xanthomonas campestris

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane monotopic membrane protein Xanthomonas campestris 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 x * 36000, SDS-PAGE, recombinant protein Xanthomonas campestris

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris Q56775
-
-

Storage Stability

Storage Stability Organism
-20°C, purified protein at 5 mg/ml is stable for at least 5 months Xanthomonas campestris
4°C, purified protein at 5 mg/ml is stable for at least 1 month at 4°C Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-mannose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol ADP-mannose can act as a mannose donor, yielding around 35% of the reaction product obtained by using GDP-mannose Xanthomonas campestris ADP + D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol
-
 ?
GDP-alpha D-glucose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol less than 15% of the reaction product obtained by using GDP-mannose Xanthomonas campestris GDP + D-Glc-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol
-
 ?
GDP-mannose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol enzyme is a nonprocessive beta-mannosyltransferase catalyzing the transfer of a mannose residue from GDP-Man to glucuronic acid-beta-1,2-mannose-alpha-1,3-glucose-beta-1,4-glucose-PP-polyisoprenyl to form the lipid-linked pentasaccharide Xanthomonas campestris GDP + D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol
-
 ?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE, recombinant protein Xanthomonas campestris

Synonyms

Synonyms Comment Organism
gumI
-
 Xanthomonas campestris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Xanthomonas campestris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20
-
 about 80% of maximum activity Xanthomonas campestris
60
-
 almost complete loss of activity Xanthomonas campestris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Xanthomonas campestris

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5
-
 Xanthomonas campestris