Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | 1D-myo-inositol 1-phosphate is neither a good substrate nor an inhibitor of MshA. Therefore the commercially available 1DL-myo-inositol 1-phosphate can be used as substrate for MshA assays without complications | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
1D-myo-inositol 3-phosphate | 37°C | Mycolicibacterium smegmatis | |
0.17 | - |
UDP-N-acetyl-D-glucosamine | 37°C | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate | Mycolicibacterium smegmatis | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, mycothiol biosynthesis | 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | - |
? | |
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, mycothiol biosynthesis | 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0QQY9 | mc2155 | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | A0QQY9 | mc2155 | - |
Storage Stability | Organism |
---|---|
4°C, 2 weeks, activity in cell extract is stable | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 1-phosphate | at 10% of the activity with 1D-myo-inositol 3-phosphate | Mycolicibacterium smegmatis | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 1-phosphate | at 10% of the activity with 1D-myo-inositol 3-phosphate | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, mycothiol biosynthesis | Mycolicibacterium smegmatis | 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | - |
? | |
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate. Little activity is obtained with myo-inositol, 1D-myo-inositol 1-phosphate, or myo-inositol 2-phosphate as the N-acetylglucosamine acceptor. 1D-myo-inositol 1-phosphate is neither a good substrate nor an inhibitor of MshA. Therfore the commercially available 1DL-myo-inositol 1-phosphate can be used as substrate for MshA assays without complications | Mycolicibacterium smegmatis | 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | - |
? | |
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, mycothiol biosynthesis | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | - |
? | |
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate. Little activity is obtained with myo-inositol, 1D-myo-inositol 1-phosphate, or myo-inositol 2-phosphate as the N-acetylglucosamine acceptor. 1D-myo-inositol 1-phosphate is neither a good substrate nor an inhibitor of MshA. Therfore the commercially available 1DL-myo-inositol 1-phosphate can be used as substrate for MshA assays without complications | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | - |
? | |
UDP-N-acetyl-D-glucosamine + myo-inositol 2-phosphate | at 10% of the activity with 1D-myo-inositol 3-phosphate | Mycolicibacterium smegmatis | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + myo-inositol 2-phosphate | at 10% of the activity with 1D-myo-inositol 3-phosphate | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MshA | - |
Mycolicibacterium smegmatis |
MSMEG0924 | - |
Mycolicibacterium smegmatis |