Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) One Shot cells | Manihot esculenta |
expressed in Escherichia coli BL21 Star (DE3) One Shot cells | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
27.5 | - |
maltotriose | at pH 7.0 and 37 °C | Arabidopsis thaliana | |
69.2 | - |
maltotriose | at pH 7.0 and 37 °C | Manihot esculenta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59400 | - |
2 * 59400, calculated from amino acid sequence | Manihot esculenta |
66000 | - |
2 * 66000, SDS-PAGE | Arabidopsis thaliana |
117000 | - |
gel filtration | Manihot esculenta |
123000 | - |
gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LV91 | - |
- |
Manihot esculenta | - |
cultivar KU50 | - |
Purification (Comment) | Organism |
---|---|
Ni HiTrap column chromatography and Superdex 75 gel filtration | Manihot esculenta |
Ni HiTrap column chromatography and Superdex 75 gel filtration | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltotriose + maltotriose | - |
Manihot esculenta | maltooligosaccharides | - |
? | |
maltotriose + maltotriose | - |
Arabidopsis thaliana | maltooligosaccharides | - |
? | |
potato amylose + glycosyl acceptor | - |
Manihot esculenta | cycloamylose | the enzyme can produce cycloamyloses in the range 16 to more than 60 glucose residues | ? | |
potato amylose + glycosyl acceptor | - |
Arabidopsis thaliana | cycloamylose | the enzyme can produce cycloamyloses in the range 1650 glucose residues | ? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 66000, SDS-PAGE | Arabidopsis thaliana |
homodimer | 2 * 59400, calculated from amino acid sequence | Manihot esculenta |
Synonyms | Comment | Organism |
---|---|---|
4alphaGTase | - |
Manihot esculenta |
4alphaGTase | - |
Arabidopsis thaliana |
DPE1 | - |
Manihot esculenta |
DPE1 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Manihot esculenta |
37 | - |
- |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
580 | - |
maltotriose | at pH 7.0 and 37 °C | Arabidopsis thaliana | |
894 | - |
maltotriose | at pH 7.0 and 37 °C | Manihot esculenta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Manihot esculenta |
6 | 8 | - |
Arabidopsis thaliana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Manihot esculenta | calculated from amino acid sequence | - |
5.1 |
Arabidopsis thaliana | calculated from amino acid sequence | - |
5.1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.9 | - |
maltotriose | at pH 7.0 and 37 °C | Manihot esculenta | |
21.6 | - |
maltotriose | at pH 7.0 and 37 °C | Arabidopsis thaliana |