Protein Variants | Comment | Organism |
---|---|---|
additional information | the amino acid substitution at Y54 or Y101 for removing their aromatic side chain increases cyclization activity, intra-molecular transglycosylation reaction, but decreases disproportionation, coupling and hydrolytic activities, inter-molecular reactions | Thermus aquaticus |
Y54A | site-directed mutagenesis, the mutant shows reduced coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54C | site-directed mutagenesis, the mutant shows reduced coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54D | site-directed mutagenesis, the mutant shows reduced coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54E | site-directed mutagenesis, the mutant shows reduced coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54F | site-directed mutagenesis, the mutant shows increased coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54G | site-directed mutagenesis, the mutant shows reduced coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54H | site-directed mutagenesis, the mutant shows increased coupling and reduced disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54I | site-directed mutagenesis, the mutant shows reduced coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54K | site-directed mutagenesis, the mutant shows unaltered coupling but reduced disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54L | site-directed mutagenesis, the mutant shows reduced coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54M | site-directed mutagenesis, the mutant shows reduced coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54N | site-directed mutagenesis, the mutant shows reduced coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54P | site-directed mutagenesis, the mutant shows reduced coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54Q | site-directed mutagenesis, the mutant shows unaltered coupling but reduced disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54R | site-directed mutagenesis, the mutant shows reduced coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54S | site-directed mutagenesis, the mutant shows reduced coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54T | site-directed mutagenesis, the mutant shows reduced coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54V | site-directed mutagenesis, the mutant shows reduced coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Y54W | site-directed mutagenesis, the mutant shows reduced coupling and disproportionation activities compared to the wild-type enzyme | Thermus aquaticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermus aquaticus | amylomaltase from Thermus aquaticus catalyzes three types of transglycosylation reaction, as well as a weak hydrolytic reaction of alpha-1,4 glucan | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus aquaticus | B7A9X4 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
disproportionation and coupling activities of wild-type and Y54 mutated enzymes, overview | Thermus aquaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(alpha-1,4 glucan)m + (alpha-1,4 glucan)n | - |
Thermus aquaticus | (alpha-1,4 glucan)m-x + (alpha-1,4 glucan)n+x | - |
r | |
additional information | amylomaltase from Thermus aquaticus catalyzes three types of transglycosylation reaction, as well as a weak hydrolytic reaction of alpha-1,4 glucan | Thermus aquaticus | ? | - |
? | |
additional information | the enzyme contains two substrate binding sites involving residues Y54 or Y101, structure and localization, overview. The binding of glucan substrate to the second glucan binding site through an interaction with the aromatic side chains of Y54 and Y101 is a trigger for the enzyme to take a completely active conformation for all four types of activity, but prevents the cyclization reaction to occur since the flexibility of the glucan is restricted by such binding. Synthesis of cycloamylose, overview | Thermus aquaticus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
amylomaltase | - |
Thermus aquaticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Thermus aquaticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Thermus aquaticus |