Cloned (Comment) | Organism |
---|---|
gene cloned an sequenced | Dictyoglomus thermophilum |
Gtase gene cloned and expressed in Escherichia coli MV1184 | Thermococcus litoralis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | - |
Thermococcus litoralis | |
Monoiodoacetic acid | - |
Thermococcus litoralis | |
Ni2+ | - |
Thermococcus litoralis | |
p-chloromercuribenzoic acid | - |
Thermococcus litoralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77880 | - |
calculated from cDNA | Thermococcus litoralis |
85000 | - |
SDS-PAGE | Thermococcus litoralis |
87000 | - |
gel filtration | Thermococcus litoralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Dictyoglomus thermophilum | - |
- |
- |
Pyrococcus furiosus | - |
- |
- |
Solanum tuberosum | - |
potato | - |
Streptococcus mitis | - |
- |
- |
Thermococcus litoralis | - |
DSM 5473 | - |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus litoralis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Solanum tuberosum | - |
leaf | - |
Arabidopsis thaliana | - |
tuber | - |
Solanum tuberosum | - |
tuber | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.2 | - |
- |
Thermococcus litoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylose + D-glucose | - |
Solanum tuberosum | low molecular mass oligosaccharides | - |
? | |
amylose + D-glucose | synthetic amylose AS-320 | Thermococcus litoralis | low molecular mass oligosaccharides | - |
? | |
amylose + maltopentaose | - |
Solanum tuberosum | cyclic alpha-1,4-glucan | cycloamylose | ? | |
amylose + maltopentaose | - |
Thermococcus litoralis | cyclic alpha-1,4-glucan | cycloamylose | ? | |
maltoheptaose + maltopentaose | - |
Arabidopsis thaliana | maltooligosaccharides | - |
? | |
maltoheptaose + maltopentaose | - |
Thermococcus litoralis | maltooligosaccharides | - |
? | |
maltohexaose + maltotriose | - |
Thermococcus litoralis | maltodextrins | - |
? | |
maltooligosaccharides | - |
Thermococcus litoralis | maltooligosaccharides + D-glucose | - |
? | |
maltopentaose + maltopentaose | - |
Thermococcus litoralis | new oligosaccharides | - |
? | |
maltose + maltose | - |
Thermococcus litoralis | maltotriose + glucose | - |
? | |
maltotetraose + maltopentaose | - |
Solanum tuberosum | maltooligosaccharides | - |
? | |
maltotetraose + maltopentaose | - |
Arabidopsis thaliana | maltooligosaccharides | - |
? | |
maltotetraose + maltopentaose | - |
Thermococcus litoralis | maltooligosaccharides | - |
? | |
maltotheptaose + maltotriose | - |
Thermococcus litoralis | maltodextrins | - |
? | |
maltotriose + glycosyl acceptor | - |
Pyrococcus furiosus | D-glucose + maltose + maltotetraose + maltopentaose + maltohexaose | - |
? | |
maltotriose + maltotriose | - |
Thermococcus litoralis | maltodextrins | - |
? | |
maltotriose + maltotriose | - |
Thermococcus litoralis | maltopentaose + maltotetraose | - |
? | |
additional information | - |
Arabidopsis thaliana | ? | - |
? | |
additional information | enzyme catalyzes not only intermolecular transglycosylation to produce linear alpha-1,4-glucan, but also intramolecular transglycosylation to produce cyclic alpha-1,4-glucan | Thermococcus litoralis | ? | - |
? | |
additional information | maltose is not a product | Solanum tuberosum | ? | - |
? | |
starch + D-glucose | - |
Solanum tuberosum | low molecular mass oligosaccharides | - |
? | |
starch + D-glucose | soluble starch | Thermococcus litoralis | low molecular mass oligosaccharides | - |
? | |
starch + glycosyl acceptor | - |
Pyrococcus furiosus | D-glucose + maltose + glucan oligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Solanum tuberosum |
monomer | 1 * 85000, SDS-PAGE | Thermococcus litoralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermococcus litoralis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 100 | - |
Thermococcus litoralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
quite thermostable, retains full activity after 3 h | Thermococcus litoralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermococcus litoralis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | completely stable at 4°C for 18 h | Thermococcus litoralis |