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Literature summary for 2.4.1.248 extracted from

  • Suzuki, R.; Terasawa, K.; Kimura, K.; Fujimoto, Z.; Momma, M.; Kobayashi, M.; Kimura, A.; Funane, K.
    Biochemical characterization of a novel cycloisomaltooligosaccharide glucanotransferase from Paenibacillus sp. 598K (2012), Biochim. Biophys. Acta, 1824, 919-924.
    View publication on PubMed
Search for more literature for 2.4.1.248

Cloned(Commentary)

Cloned (Comment) Organism
gene cit, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Paenibacillus sp.
gene cit, expression in Escherichia coli Niallia circulans

Protein Variants

Protein Variants Comment Organism
D144A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Paenibacillus sp.
D144N site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Paenibacillus sp.
D264A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Paenibacillus sp.
D269A site-directed mutagenesis, the mutant is inactive Paenibacillus sp.
D269N site-directed mutagenesis, the mutant is almost inactive Paenibacillus sp.
D662A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Paenibacillus sp.
E341A site-directed mutagenesis, the mutant is inactive Paenibacillus sp.
E341Q site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Niallia circulans P94286 gene cit
-
Niallia circulans T-3040 P94286 gene cit
-
Paenibacillus sp. G9MBW2 gene cit
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information isomaltotetraose is the smallest substrate for the CITase, and major product of recombinant CITase-598K is cycloisomaltoheptaose Paenibacillus sp. ?
-
 ?
additional information isomaltotetraose is the smallest substrate for the CITase, and major product of recombinant CITase-T3040 is cycloisomaltooctaose Niallia circulans ?
-
 ?
additional information isomaltotetraose is the smallest substrate for the CITase, and major product of recombinant CITase-T3040 is cycloisomaltooctaose Niallia circulans T-3040 ?
-
 ?

Synonyms

Synonyms Comment Organism
CITase
-
 Niallia circulans
CITase
-
 Paenibacillus sp.
CITase-598K
-
 Paenibacillus sp.
CITase-T3040
-
 Niallia circulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50 assay at Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 recombinant enzyme, stable up to Niallia circulans
50
-
 recombinant enzyme, stable up to Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 8
-
 Niallia circulans
5.5 8
-
 Paenibacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 stable at Niallia circulans
5 9 stable at Paenibacillus sp.

General Information

General Information Comment Organism
evolution CITase is a member of the glycoside hydrolase family 66, GH66 Niallia circulans
evolution CITase is a member of the glycoside hydrolase family 66, GH66 Paenibacillus sp.
additional information catalytically important residues of CITase-598K are Asp144, Asp269, and Glu341 Paenibacillus sp.