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Literature summary for 2.4.1.248 extracted from

  • Funane, K.; Kawabata, Y.; Suzuki, R.; Kim, Y.M.; Kang, H.K.; Suzuki, N.; Fujimoto, Z.; Kimura, A.; Kobayashi, M.
    Deletion analysis of regions at the C-terminal part of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040 (2011), Biochim. Biophys. Acta, 1814, 428-434.
    View publication on PubMed
Search for more literature for 2.4.1.248

Protein Variants

Protein Variants Comment Organism
additional information construction of CITase deletion mutants, 15 deletion mutant enzymes. M123DELTA (R4-deleted), MDELTA234 (R1-deleted), and MDELTA23DELTA (R1/R4-deleted) catalyze cycloisomaltooligosaccharide synthesis, but other mutants are inactive. M123DELTA, MDELTA234, and MDELTA23DELTA increase their Km values against dextran 40. The wild-type enzyme and M123DELTA produced cycloisomaltooligosaccharide-8 predominantly, but MDELTA234 and MDELTA23DELTA lose cycloisomaltooligosaccharide-8 production specificity. The kcat values of MDELTA234 and MDELTA23DELTA decrease, and these mutants show narrowed temperature and pH stability ranges, product size distribution of mutants, overview Niallia circulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics for wild-type and deletion mutant enzymes, overview Niallia circulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Niallia circulans CITase catalyzes the synthesis of cycloisomaltooligosaccharides with 7-17 glucose units from dextran ?
-
 ?
additional information Niallia circulans T-3040 CITase catalyzes the synthesis of cycloisomaltooligosaccharides with 7-17 glucose units from dextran ?
-
 ?

Organism

Organism UniProt Comment Textmining
Niallia circulans P94286
-
-
Niallia circulans T-3040 P94286
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information CITase catalyzes the synthesis of cycloisomaltooligosaccharides with 7-17 glucose units from dextran Niallia circulans ?
-
 ?
additional information CITase catalyzes the synthesis of cycloisomaltooligosaccharides with 7-17 glucose units from dextran Niallia circulans T-3040 ?
-
 ?

Subunits

Subunits Comment Organism
More CITase contains an N-terminal conserved region of Ser1-Gly403, an insertion region R1 of Tyr404-Tyr492, two conserved regions R2 of Glu493-Ser596 and R3 Gly597-Met700, and a C-terminal variable region R4 of Lys701-Ser934 Niallia circulans

Synonyms

Synonyms Comment Organism
CITase
-
 Niallia circulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 mutant MDELTA23DELTA Niallia circulans
30
-
 mutant MDELTA234 Niallia circulans
40
-
 wild-type enzyme and mutant M123DELTA Niallia circulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 mutant MDELTA23DELTA, stable up to Niallia circulans
40
-
 mutant MDELTA234, stable up to Niallia circulans
50
-
 wild-type enzyme and mutant M123DELTA, stable up to Niallia circulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 wild-type enzyme and mutant M123DELTA Niallia circulans
6
-
 mutant MDELTA234 Niallia circulans
6.5
-
 mutant MDELTA23DELTA Niallia circulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 wild-type enzyme and mutant M123DELTA, stable at Niallia circulans
5.5 10 mutant MDELTA234, stable at Niallia circulans
6 8.5 mutant MDELTA23DELTA, stable at Niallia circulans

General Information

General Information Comment Organism
evolution CITase belongs to glycoside hydrolase family 66, GH6 Niallia circulans
malfunction 15 deletion mutant enzymes. M123DELTA (R4-deleted), MDELTA234 (R1-deleted), and MDELTA23DELTA (R1/R4-deleted) catalyze cycloisomaltooligosaccharide synthesis, but other mutants are inactive. M123DELTA, MDELTA234, and MDELTA23DELTA increase their Km values against dextran 40. The wild-type enzyme and M123DELTA produced cycloisomaltooligosaccharide-8 predominantly, but MDELTA234 and MDELTA23DELTA lose cycloisomaltooligosaccharide-8 production specificity. The kcat values of MDELTA234 and MDELTA23DELTA decrease, and these mutants show narrowed temperature and pH stability ranges Niallia circulans