Application | Comment | Organism |
---|---|---|
synthesis | suitable catalyst for practical syntheses of oligosaccharides | Lachnoclostridium phytofermentans |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Lachnoclostridium phytofermentans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
L-rhamnose | - |
Lachnoclostridium phytofermentans | |
7.9 | - |
D-galactosyl-beta-1,4-L-rhamnose | - |
Lachnoclostridium phytofermentans | |
78 | - |
L-Mannose | - |
Lachnoclostridium phytofermentans | |
220 | - |
L-Lyxose | - |
Lachnoclostridium phytofermentans | |
290 | - |
2-deoxy-D-glucose | - |
Lachnoclostridium phytofermentans | |
460 | - |
D-glucose | - |
Lachnoclostridium phytofermentans | |
610 | - |
D-galactose | - |
Lachnoclostridium phytofermentans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | proteins are predicted not to possess N-terminal signal peptides | Lachnoclostridium phytofermentans | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
SDS-PAGE | Lachnoclostridium phytofermentans |
84230 | - |
calculated | Lachnoclostridium phytofermentans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lachnoclostridium phytofermentans | A9KHK4 | bacterium possesses three GalHexNAcP homologous genes, cphy1920 gene; anaerobic cellulolytic bacterium | - |
Purification (Comment) | Organism |
---|---|
- |
Lachnoclostridium phytofermentans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + alpha-D-galactose 1-phosphate | when D-glucose derivatives are used as acceptors, reaction products are beta-1,3-galactosides | Lachnoclostridium phytofermentans | D-galactosyl-beta-1,3-2-deoxy-D-glucose + phosphate | - |
r | |
alpha-D-galactose 1-phosphate + L-rhamnose | highest activity | Lachnoclostridium phytofermentans | D-galactosyl-beta-1,4-L-rhamnose + phosphate | - |
r | |
D-galactose + alpha-D-galactose 1-phosphate | - |
Lachnoclostridium phytofermentans | D-galactosyl-beta-1,3-D-galactose + phosphate | - |
r | |
D-glucose + alpha-D-galactose 1-phosphate | when D-glucose derivatives are used as acceptors, reaction products are beta-1,3-galactosides | Lachnoclostridium phytofermentans | D-galactosyl-beta-1,3-D-glucose + phosphate | - |
r | |
L-lyxose + alpha-D-galactose 1-phosphate | - |
Lachnoclostridium phytofermentans | beta-D-galactosyl-(1->4)-L-lyxose + phosphate | - |
r | |
L-mannose + alpha-D-galactose 1-phosphate | - |
Lachnoclostridium phytofermentans | D-galactosyl-beta-1,4-L-mannose + phosphate | - |
r | |
additional information | phosphorylyzes neither galacto-N-biose (D-Gal-beta1,3-D-GalNAc) nor lacto-N-biose I(D-Gal-beta1,3-D-GlcNAc), thus indicating that Cphy1920 protein is not GalHexNAcP | Lachnoclostridium phytofermentans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cphy1920 protein | - |
Lachnoclostridium phytofermentans |
D-galactosyl-beta-1,4-L-rhamnose phosphorylase | - |
Lachnoclostridium phytofermentans |
GalRhaP | - |
Lachnoclostridium phytofermentans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
protein is thermostable up to 37°C | Lachnoclostridium phytofermentans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 8 | 2-deoxy-D-glucose | - |
Lachnoclostridium phytofermentans | |
24 | - |
D-galactose | - |
Lachnoclostridium phytofermentans | |
45 | - |
D-galactosyl-beta-1,4-L-rhamnose | - |
Lachnoclostridium phytofermentans | |
74 | - |
L-rhamnose | - |
Lachnoclostridium phytofermentans | |
85 | - |
D-glucose | - |
Lachnoclostridium phytofermentans | |
120 | - |
L-Lyxose | - |
Lachnoclostridium phytofermentans | |
170 | - |
L-Mannose | - |
Lachnoclostridium phytofermentans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | optimum activity at neutral pH | Lachnoclostridium phytofermentans |