Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is a useful thermostable enzyme particularly for the production of the trehalose analogue containing a mannose that acts as the inhibitor of intestinal sucrase | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 35% loss of activity | Thermotoga maritima | |
dimethylformamide | 5 mM, 79% loss of activity | Thermotoga maritima | |
DMSO | 5 mM, 89% loss of activity | Thermotoga maritima | |
ethanol | 5 mM, 47% loss of activity | Thermotoga maritima | |
methanol | 5 mM, 36% loss of activity | Thermotoga maritima | |
Mn2+ | 5 mM, 82% loss of activity | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
UDP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
1.7 | - |
GDP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
2.3 | - |
ADP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
40 | - |
ADP | pH 6.0, 60°C | Thermotoga maritima | |
54 | - |
GDP | pH 6.0, 60°C | Thermotoga maritima | |
71 | - |
UDP | pH 6.0, 60°C | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 5 mM, 1.2fold activation | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
SDS-PAGE | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Thermotoga maritima DSM 3109 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-alpha-D-glucose + D-glucose | - |
Thermotoga maritima | alpha,alpha-trehalose + ADP | - |
r | |
ADP-alpha-D-glucose + D-glucose | - |
Thermotoga maritima DSM 3109 | alpha,alpha-trehalose + ADP | - |
r | |
alpha,alpha-trehalose + ADP | both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order | Thermotoga maritima | ADP-alpha-D-glucose + D-glucose | - |
r | |
alpha,alpha-trehalose + ADP | both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order | Thermotoga maritima DSM 3109 | ADP-alpha-D-glucose + D-glucose | - |
r | |
alpha,alpha-trehalose + CDP | both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order | Thermotoga maritima | CDP-alpha-D-glucose + D-glucose | - |
r | |
alpha,alpha-trehalose + GDP | both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order | Thermotoga maritima | GDP-alpha-D-glucose + D-glucose | - |
? | |
alpha,alpha-trehalose + GDP | both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order | Thermotoga maritima DSM 3109 | GDP-alpha-D-glucose + D-glucose | - |
? | |
alpha,alpha-trehalose + UDP | both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order | Thermotoga maritima | UDP-alpha-D-glucose + D-glucose | - |
? | |
CDP-alpha-D-glucose + D-glucose | - |
Thermotoga maritima | alpha,alpha-trehalose + CDP | - |
r | |
GDP-alpha-D-glucose + D-glucose | - |
Thermotoga maritima | alpha,alpha-trehalose + GDP | - |
r | |
additional information | the enzyme does not utilize galactose as an acceptor | Thermotoga maritima | ? | - |
? | |
additional information | the enzyme does not utilize galactose as an acceptor | Thermotoga maritima DSM 3109 | ? | - |
? | |
UDP-alpha-D-glucose + D-fructose | the enzyme employs a glucose acceptor with the highest preference and other monosaccharides (D-fructose, D-mannose) with less affinities acceptors. The trehalose analogue containing mannose is produced with approximately 70% of trehalose production and a two-fold greater amount than the analogue containing a fructose for the 12-24 h reaction | Thermotoga maritima | ? | - |
r | |
UDP-alpha-D-glucose + D-glucose | the purified soluble enzyme, as well as the glutathione-S-transferase-fusion enzyme, show the catalytic specificity for synthesizing trehalose from UDP-alpha-D-glucose as the donor and glucose as the acceptor. The enzyme employs a glucose acceptor with the highest preference and other monosaccharides (D-fructose, D-mannose) with less affinities acceptors. Both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order | Thermotoga maritima | alpha,alpha-trehalose + UDP | - |
r | |
UDP-alpha-D-glucose + D-mannose | the enzyme employs a glucose acceptor with the highest preference and other monosaccharides (D-fructose, D-mannose) with less affinities acceptors. The trehalose analogue containing mannose is produced with approximately 70% of trehalose production and a two-fold greater amount than the analogue containing a fructose for the 12-24 h reaction | Thermotoga maritima | ? | - |
r | |
UDP-alpha-D-glucose + D-mannose | the enzyme employs a glucose acceptor with the highest preference and other monosaccharides (D-fructose, D-mannose) with less affinities acceptors. The trehalose analogue containing mannose is produced with approximately 70% of trehalose production and a two-fold greater amount than the analogue containing a fructose for the 12-24 h reaction | Thermotoga maritima DSM 3109 | ? | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
TmTreT | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 70 | 35°C: about 40% of maximal activity, 70°C: about 70% of maximal activity | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
2 h, activity of the the glutathione-S-transferase-fusion enzyme is fully maintained | Thermotoga maritima |
60 | - |
2 h, 10% decrease of the activity of the the glutathione-S-transferase-fusion enzyme, activity of the soluble enzyme is decreased by 35% | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.1 | - |
UDP | pH 6.0, 60°C | Thermotoga maritima | |
9.7 | - |
GDP | pH 6.0, 60°C | Thermotoga maritima | |
11.1 | - |
ADP | pH 6.0, 60°C | Thermotoga maritima | |
20 | - |
ADP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
25 | - |
UDP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
34 | - |
GDP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | pH 4.5: about 45% of maximal activity, pH 8.0: about 40% of maximal activity | Thermotoga maritima |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | activity is relatively stable in the wide pH range of 5.0-10.0 | Thermotoga maritima |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
UDP | pH 6.0, 60°C | Thermotoga maritima | |
0.2 | - |
GDP | pH 6.0, 60°C | Thermotoga maritima | |
0.3 | - |
ADP | pH 6.0, 60°C | Thermotoga maritima | |
9 | - |
ADP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
15 | - |
UDP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
20 | - |
GDP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima |