Application | Comment | Organism |
---|---|---|
synthesis | the enzyme may be useful for the regeneration of NDP-alpha-D-glucose from NDP, especially for ADP-alpha-D-glucose from ADP, with trehalose as a glucose resource | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.08 | - |
GDP-alpha-D-glucose | pH 6.0, 60°C | Pyrococcus horikoshii | |
2.46 | - |
UDP-alpha-D-glucose | pH 6.0, 60°C | Pyrococcus horikoshii | |
3.2 | - |
ADP-alpha-D-glucose | pH 6.0, 60°C | Pyrococcus horikoshii | |
14.19 | - |
ADP | pH 6.0, 60°C | Pyrococcus horikoshii | |
23.77 | - |
GDP | pH 6.0, 60°C | Pyrococcus horikoshii | |
25.24 | - |
alpha,alpha-trehalose | pH 6.0, 60°C | Pyrococcus horikoshii | |
29.54 | - |
UDP | pH 6.0, 60°C | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-alpha-D-glucose + D-glucose | GDP-alpha-D-glucose is the most favored in terms of reaction specificity, kcat/Km. UDP-alpha-D-glucose and ADP-alpha-D-glucose are employed with less preferences. The enzyme reversely cleaves alpha,alpha-trehalose to transfer the glucosyl moiety to various NDPs, efficiently producing NDP-alpha-D-glucose. Although ADP-alpha-D-glucose is the least favorable donor, the counterpart, ADP, is the most favorable acceptor for the reverse synthesis of NDP-alpha-D-glucose in kcat/Km. GDP and UDP are less preferred, compared to ADP | Pyrococcus horikoshii | alpha,alpha-trehalose + ADP | - |
r | |
alpha,alpha-trehalose + ADP | the enzyme reversely cleaves alpha,alpha-trehalose to transfer the glucosyl moiety to various NDPs, efficiently producing NDP-alpha-D-glucose. Although ADP-alpha-D-glucose is the least favorable donor, the counterpart, ADP, is the most favorable acceptor for the reverse synthesis of NDP-alpha-D-glucose in kcat/Km. GDP and UDP are less preferred, compared to ADP | Pyrococcus horikoshii | ADP-alpha-D-glucose + D-glucose | - |
r | |
alpha,alpha-trehalose + GDP | the enzyme reversely cleaves alpha,alpha-trehalose to transfer the glucosyl moiety to various NDPs, efficiently producing NDP-alpha-D-glucose. Although ADP-alpha-D-glucose is the least favorable donor, the counterpart, ADP, is the most favorable acceptor for the reverse synthesis of NDP-alpha-D-glucose in kcat/Km. GDP and UDP are less preferred, compared to ADP | Pyrococcus horikoshii | GDP-alpha-D-glucose + D-glucose | - |
r | |
alpha,alpha-trehalose + UDP | the enzyme reversely cleaves alpha,alpha-trehalose to transfer the glucosyl moiety to various NDPs, efficiently producing NDP-alpha-D-glucose. Although ADP-alpha-D-glucose is the least favorable donor, the counterpart, ADP, is the most favorable acceptor for the reverse synthesis of NDP-alpha-D-glucose in kcat/Km. GDP and UDP are less preferred, compared to ADP | Pyrococcus horikoshii | UDP-alpha-D-glucose + D-glucose | - |
r | |
GDP-alpha-D-glucose + D-glucose | GDP-alpha-D-glucose is the most favored in terms of reaction specificity, kcat/Km. UDP-alpha-D-glucose and ADP-alpha-D-glucose are employed with less preferences. The enzyme reversely cleaves alpha,alpha-trehalose to transfer the glucosyl moiety to various NDPs, efficiently producing NDP-alpha-D-glucose. Although ADP-alpha-D-glucose is the least favorable donor, the counterpart, ADP, is the most favorable acceptor for the reverse synthesis of NDP-alpha-D-glucose in kcat/Km. GDP and UDP are less preferred, compared to ADP | Pyrococcus horikoshii | alpha,alpha-trehalose + GDP | - |
r | |
UDP-alpha-D-glucose + D-glucose | GDP-alpha-D-glucose is the most favored in terms of reaction specificity, kcat/Km. UDP-alpha-D-glucose and ADP-alpha-D-glucose are employed with less preferences. The enzyme reversely cleaves alpha,alpha-trehalose to transfer the glucosyl moiety to various NDPs, efficiently producing NDP-alpha-D-glucose. Although ADP-alpha-D-glucose is the least favorable donor, the counterpart, ADP, is the most favorable acceptor for the reverse synthesis of NDP-alpha-D-glucose in kcat/Km. GDP and UDP are less preferred, compared to ADP | Pyrococcus horikoshii | alpha,alpha-trehalose + UDP | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TreT | - |
Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
ADP-alpha-D-glucose | pH 6.0, 60°C | Pyrococcus horikoshii | |
0.53 | - |
UDP-alpha-D-glucose | pH 6.0, 60°C | Pyrococcus horikoshii | |
0.54 | - |
GDP-alpha-D-glucose | pH 6.0, 60°C | Pyrococcus horikoshii | |
1.2 | - |
alpha,alpha-trehalose | pH 6.0, 60°C | Pyrococcus horikoshii | |
1.5 | - |
UDP | pH 6.0, 60°C | Pyrococcus horikoshii | |
1.7 | - |
ADP | pH 6.0, 60°C | Pyrococcus horikoshii | |
1.7 | - |
GDP | pH 6.0, 60°C | Pyrococcus horikoshii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
UDP | pH 6.0, 60°C | Pyrococcus horikoshii | |
0.05 | - |
alpha,alpha-trehalose | pH 6.0, 60°C | Pyrococcus horikoshii | |
0.07 | - |
GDP | pH 6.0, 60°C | Pyrococcus horikoshii | |
0.12 | - |
ADP | pH 6.0, 60°C | Pyrococcus horikoshii | |
0.14 | - |
ADP-alpha-D-glucose | pH 6.0, 60°C | Pyrococcus horikoshii | |
0.22 | - |
UDP-alpha-D-glucose | pH 6.0, 60°C | Pyrococcus horikoshii | |
0.26 | - |
GDP-alpha-D-glucose | pH 6.0, 60°C | Pyrococcus horikoshii |