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Literature summary for 2.4.1.245 extracted from

  • Kouril, T.; Zaparty, M.; Marrero, J.; Brinkmann, H.; Siebers, B.
    A novel trehalose synthesizing pathway in the hyperthermophilic crenarchaeon Thermoproteus tenax: the unidirectional TreT pathway (2008), Arch. Microbiol., 190, 355-369.
    View publication on PubMed
Search for more literature for 2.4.1.245

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermoproteus tenax

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Thermoproteus tenax

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 UDP-glucose in 50 mM HEPES/KOH (pH 7.0), with 20 mM MgCl2, at 70°C Thermoproteus tenax
3.57
-
 D-glucose in 50 mM HEPES/KOH (pH 7.0), with 20 mM MgCl2, at 70°C Thermoproteus tenax

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Thermoproteus tenax

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44400
-
 subunit, calculated from amino acid sequence Thermoproteus tenax
47000
-
 subunit, SDS-PAGE Thermoproteus tenax
100000
-
 native enzyme, gel filtration Thermoproteus tenax

Organism

Organism UniProt Comment Textmining
Thermoproteus tenax
-
-
-
Thermoproteus tenax Kra 1 / DSM 2078
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography Thermoproteus tenax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + D-glucose 10% activity with ADP-glucose compared to UDP-glucose Thermoproteus tenax ADP + alpha,alpha-1,1-trehalose
-
 r
ADP-alpha-D-glucose + D-glucose 10% activity with ADP-glucose compared to UDP-glucose Thermoproteus tenax Kra 1 / DSM 2078 ADP + alpha,alpha-1,1-trehalose
-
 r
additional information no activity with maltose and glucose 1-phosphate Thermoproteus tenax ?
-
 ?
additional information no activity with maltose and glucose 1-phosphate Thermoproteus tenax Kra 1 / DSM 2078 ?
-
 ?
UDP-glucose + D-glucose clear preference for UDP-glucose over ADP-glucose Thermoproteus tenax alpha,alpha-1,1-trehalose + UDP
-
 r
UDP-glucose + D-glucose clear preference for UDP-glucose over ADP-glucose Thermoproteus tenax Kra 1 / DSM 2078 alpha,alpha-1,1-trehalose + UDP
-
 r

Synonyms

Synonyms Comment Organism
trehalose glycosyltransferring synthase
-
 Thermoproteus tenax
TreT
-
 Thermoproteus tenax