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Literature summary for 2.4.1.245 extracted from
- TreT, a novel trehalose glycosyltransferring synthase of the hyperthermophilic archaeon Thermococcus litoralis (2004), J. Biol. Chem., 279, 47890-47897.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, with His-tag | Thermococcus litoralis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.14 | - |
ADP-glucose | 90°C, pH 6.5 | Thermococcus litoralis |  |
| 6.2 | - |
D-glucose | 90°C, pH 6.5 | Thermococcus litoralis | |
| 11.5 | - |
alpha,alpha-1,1-trehalose | 90°C, pH 6.5 | Thermococcus litoralis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 91% of the activity with Mg2+ | Thermococcus litoralis | |
| Co2+ | 72% of the activity with Mg2+ | Thermococcus litoralis | |
| Mg2+ | required | Thermococcus litoralis | |
| Mn2+ | 78% of the activity with Mg2+ | Thermococcus litoralis | |
| Ni2+ | 57% of the activity with Mg2+ | Thermococcus litoralis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
2 * 48146, calculated, 2 * 48000, SDS-PAGE | Thermococcus litoralis |
| 48146 | - |
2 * 48146, calculated, 2 * 48000, SDS-PAGE | Thermococcus litoralis |
| 104000 | - |
PAGE | Thermococcus litoralis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus litoralis | - |
induced by trehalose and maltose in growth medium | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-alpha-D-glucose + D-glucose | - |
Thermococcus litoralis | ADP + alpha,alpha-1,1-trehalose | - |
r | |
| alpha,alpha-1,1-trehalose + ADP | - |
Thermococcus litoralis | ADP-glucose + D-glucose | - |
r | |
| GDP-glucose + D-glucose | 5% of the efficiency with ADP-glucose | Thermococcus litoralis | alpha,alpha-1,1-trehalose + GDP | - |
r | |
| additional information | reaction is reversible, formation of trehalose is favored by rate of reaction and equilibrium. No substrate: glucose 1-phosphate, glucose 6-phosphate, fructose, mannose, galactose, xylose, 2-deoxyglucose, glucosamine, alpha-methylglucoside, sorbitol | Thermococcus litoralis | ? | - |
? | |
| UDP-glucose + D-glucose | 6% of the efficiency with ADP-glucose | Thermococcus litoralis | alpha,alpha-1,1-trehalose + UDP | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 48146, calculated, 2 * 48000, SDS-PAGE | Thermococcus litoralis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TreT | - |
Thermococcus litoralis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Thermococcus litoralis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
65% residual activity | Thermococcus litoralis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Thermococcus litoralis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
13% residual activity | Thermococcus litoralis |
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Release 2023.1 (January 2023)
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