Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, with His-tag | Thermococcus litoralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.14 | - |
ADP-glucose | 90°C, pH 6.5 | Thermococcus litoralis | |
6.2 | - |
D-glucose | 90°C, pH 6.5 | Thermococcus litoralis | |
11.5 | - |
alpha,alpha-1,1-trehalose | 90°C, pH 6.5 | Thermococcus litoralis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 91% of the activity with Mg2+ | Thermococcus litoralis | |
Co2+ | 72% of the activity with Mg2+ | Thermococcus litoralis | |
Mg2+ | required | Thermococcus litoralis | |
Mn2+ | 78% of the activity with Mg2+ | Thermococcus litoralis | |
Ni2+ | 57% of the activity with Mg2+ | Thermococcus litoralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48146, calculated, 2 * 48000, SDS-PAGE | Thermococcus litoralis |
48146 | - |
2 * 48146, calculated, 2 * 48000, SDS-PAGE | Thermococcus litoralis |
104000 | - |
PAGE | Thermococcus litoralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus litoralis | - |
induced by trehalose and maltose in growth medium | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-alpha-D-glucose + D-glucose | - |
Thermococcus litoralis | ADP + alpha,alpha-1,1-trehalose | - |
r | |
alpha,alpha-1,1-trehalose + ADP | - |
Thermococcus litoralis | ADP-glucose + D-glucose | - |
r | |
GDP-glucose + D-glucose | 5% of the efficiency with ADP-glucose | Thermococcus litoralis | alpha,alpha-1,1-trehalose + GDP | - |
r | |
additional information | reaction is reversible, formation of trehalose is favored by rate of reaction and equilibrium. No substrate: glucose 1-phosphate, glucose 6-phosphate, fructose, mannose, galactose, xylose, 2-deoxyglucose, glucosamine, alpha-methylglucoside, sorbitol | Thermococcus litoralis | ? | - |
? | |
UDP-glucose + D-glucose | 6% of the efficiency with ADP-glucose | Thermococcus litoralis | alpha,alpha-1,1-trehalose + UDP | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 48146, calculated, 2 * 48000, SDS-PAGE | Thermococcus litoralis |
Synonyms | Comment | Organism |
---|---|---|
TreT | - |
Thermococcus litoralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermococcus litoralis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
65% residual activity | Thermococcus litoralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Thermococcus litoralis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
13% residual activity | Thermococcus litoralis |