Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.243 extracted from

  • Fujishima, M.; Sakai, H.; Ueno, K.; Takahashi, N.; Onodera, S.; Benkeblia, N.; Shiomi, N.
    Purification and characterization of a fructosyltransferase from onion bulbs and its key role in the synthesis of fructo-oligosaccharides in vivo (2005), New Phytol., 165, 513-524.
    View publication on PubMed
Search for more literature for 2.4.1.243

Activating Compound

Activating Compound Comment Organism Structure
sodium deoxycholate 1 mM, 209% of initial activity Allium cepa
Triton X-100 1 mM, 160% of initial activity Allium cepa
Tween-80 1mM, 171% of initial activity Allium cepa

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, no residual activity Allium cepa
Hg2+ 1 mM, no residual activity Allium cepa
p-chloromercuribenzoate 0.1 mM, 2.5% residual activity Allium cepa
Sodium dodecyl sulfate 1 mM, 48% residual activity Allium cepa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
88
-
 1F(1-beta-D-fructofuranosyl)sucrose pH 5.7, 30°C Allium cepa
310
-
 1F(1-beta-D-fructofuranosyl)2 sucrose pH 5.7, 30°C Allium cepa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 52000, x * 25000, SDS-PAGE Allium cepa
52000
-
 x * 52000, x * 25000, SDS-PAGE Allium cepa
66000
-
 gel filtration Allium cepa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Allium cepa plays an important role in the synthesis of inulin and inulinneo-series fructo-oligosaccharides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Allium cepa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Allium cepa

Source Tissue

Source Tissue Comment Organism Textmining
bulb
-
 Allium cepa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26.54
-
 pH 5.68, 30°C Allium cepa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1F(1-beta-D-fructofuranosyl)2 sucrose + sucrose i.e. nystose Allium cepa 1F,6G-di-beta-D-fructofuranosylsucrose + ?
-
 ?
1F(1-beta-D-fructofuranosyl)3 sucrose + sucrose i.e. fructosyl nystose Allium cepa 1F,6G-di-beta-D-fructofuranosylsucrose + ?
-
 ?
1F(1-beta-D-fructofuranosyl)sucrose + sucrose i.e. 1-kestose Allium cepa 1F,6G-di-beta-D-fructofuranosylsucrose + ?
-
 ?
additional information plays an important role in the synthesis of inulin and inulinneo-series fructo-oligosaccharides Allium cepa ?
-
 ?
neokestose + sucrose
-
 Allium cepa 1F,6G-di-beta-D-fructofuranosylsucrose + ?
-
 ?
sucrose + sucrose
-
 Allium cepa 1F,6G-di-beta-D-fructofuranosylsucrose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 52000, x * 25000, SDS-PAGE Allium cepa
More N-terminal amino acid sequence of 52000 Da polypeptide and of 25000 Da polypeptide Allium cepa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 stable for at least 15 min Allium cepa
50
-
 inactivation Allium cepa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7
-
-
 Allium cepa

pH Range

pH Minimum pH Maximum Comment Organism
5.4 6.3 more than 80% of maximum activity within this range Allium cepa

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.3 6.3 30°C, stable for 30 min Allium cepa