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Literature summary for 2.4.1.239 extracted from

  • Shute, J.L.; Jourdan, P.S.; Mansell, R.L.
    UDP-glucose:glucosyltransferase activity involved in the biosynthesis of flavonol triglucosides in Pisum sativum L. seedlings (1979), Z. Naturforsch. C, 34, 738-741.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pisum sativum involved in the biosynthesis of flavonol triglucosides ?
-
?

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
var. Alaska
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Reaction

Reaction Comment Organism Reaction ID
UDP-glucose + a flavonol 3-O-beta-D-glucoside = UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside one of three specific glucosyltransferases in pea, Pisum sativum, that successively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside, see also EC 2.4.1.91, flavonol 3-O-glucosyltransferase and EC 2.4.1.240, flavonol-3-O-glycoside glucosyltransferase, TDP-glucose can replace UDP-glucose as the glucose donor but the reaction proceeds more slowly Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
leaf young leaf tissues have much higher levels of glucosyltransferase activity than the petioles and internodes Pisum sativum
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seedling young, light-grown, much higher levels of glucosyltransferase activity in young leaf tissues than in petioles and internodes Pisum sativum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information involved in the biosynthesis of flavonol triglucosides Pisum sativum ?
-
?
additional information glucosylation of kaempferol and quercetin in the 3-position to form the 3-O-triglucoside, reaction proceeds from the aglycone via the mono- and diglucoside intermediates, this reaction sequence is probably associated with three distinct enzyme activities Pisum sativum ?
-
?
UDP-glucose + kaempferol 3-O-beta-D-glucoside glucosylates kaempferol monoglucoside and quercetin monoglucoside equally Pisum sativum UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
?
UDP-glucose + quercetin 3-O-beta-D-glucoside glucosylates kaempferol monoglucoside and quercetin monoglucoside equally Pisum sativum UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
assay at Pisum sativum