Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pisum sativum | involved in the biosynthesis of flavonol triglucosides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
var. Alaska | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-glucose + a flavonol 3-O-beta-D-glucoside = UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside | one of three specific glucosyltransferases in pea, Pisum sativum, that successively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside, see also EC 2.4.1.91, flavonol 3-O-glucosyltransferase and EC 2.4.1.240, flavonol-3-O-glycoside glucosyltransferase, TDP-glucose can replace UDP-glucose as the glucose donor but the reaction proceeds more slowly | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young leaf tissues have much higher levels of glucosyltransferase activity than the petioles and internodes | Pisum sativum | - |
seedling | young, light-grown, much higher levels of glucosyltransferase activity in young leaf tissues than in petioles and internodes | Pisum sativum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | involved in the biosynthesis of flavonol triglucosides | Pisum sativum | ? | - |
? | |
additional information | glucosylation of kaempferol and quercetin in the 3-position to form the 3-O-triglucoside, reaction proceeds from the aglycone via the mono- and diglucoside intermediates, this reaction sequence is probably associated with three distinct enzyme activities | Pisum sativum | ? | - |
? | |
UDP-glucose + kaempferol 3-O-beta-D-glucoside | glucosylates kaempferol monoglucoside and quercetin monoglucoside equally | Pisum sativum | UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside | - |
? | |
UDP-glucose + quercetin 3-O-beta-D-glucoside | glucosylates kaempferol monoglucoside and quercetin monoglucoside equally | Pisum sativum | UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Pisum sativum |