Cloned (Comment) | Organism |
---|---|
functionally expressed in Escherichia coli as His- and GST-tagged protein, UGT71F1 | Beta vulgaris |
functionally expressed in Escherichia coli as His- and GST-tagged protein, UGT73A4 | Beta vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4,4'-diisothiocyanostilbene-2,2'-disulfonic acid | 0.01 mM, UGT71F1; 0.01 mM, UGT73A4 | Beta vulgaris | |
Co2+ | complete inhibition near 0.04 mM, UGT71F1; complete inhibition near 0.04 mM, UGT73A4 | Beta vulgaris | |
Cu2+ | complete inhibition near 0.04 mM, UGT71F1; complete inhibition near 0.04 mM, UGT73A4 | Beta vulgaris | |
p-chloromercuribenzene sulfonic acid | UGT71F1, 0.01mM, 22% inhibition; UGT73A4 activity is completely inhibited at 0.01mM | Beta vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
quercetin | UGT71F1 | Beta vulgaris | |
0.011 | - |
quercetin | UGT73A4 | Beta vulgaris | |
0.0192 | - |
apigenin | UGT73A4 | Beta vulgaris | |
0.0268 | - |
apigenin | UGT71F1 | Beta vulgaris | |
0.029 | - |
betanidin | UGT71F1 | Beta vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for bivalent cations, UGT71F1 | Beta vulgaris | |
additional information | no requirement for bivalent cations, UGT73A4 | Beta vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54070 | - |
calculated from sequence, UGT73A4 | Beta vulgaris |
54390 | - |
calculated from sequence, UGT71F1 | Beta vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Beta vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant UGT71F1 | Beta vulgaris |
recombinant UGT73A4 | Beta vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
UGT71F1 can not be frozen nor stored at 4°C as 50% activity is lost within 24 h | Beta vulgaris |
UGT73A4 can be stored at 4°C or frozen at -20°C or -80°C for several months without requiring addition of stabilizing agents such as bovine serum albumin or glycerol | Beta vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + kaempferol | 81% of the activity with quercetin, UGT73A4 | Beta vulgaris | UDP + kaempferol 7-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + kaempferol | 17% of the activity with quercetin, UGT71F1 | Beta vulgaris | UDP + kaempferol 3-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + quercetin | UGT71F1 | Beta vulgaris | UDP + quercetin 7-O-beta-D-glucoside + quercetin 3-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + quercetin | 4'-OH glycosylation is preferred over 7-OH glycosylation, UGT73A4 | Beta vulgaris | UDP + quercetin 7-O-beta-D-glucoside + quercetin 4'-O-beta-D-glucoside | - |
? | |
UDP-glucose + apigenin | 40% of the activity with quercetin, UGT71F1 | Beta vulgaris | UDP + apigenin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + apigenin | 90% of the activity with quercetin, UGT73A4 | Beta vulgaris | UDP + apigenin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + betanidin | 28% of the activity with quercetin, UGT71F1 | Beta vulgaris | UDP + betanidin 5-O-beta-D-glucoside + betanidin 6-O-beta-D-glucoside | - |
? | |
UDP-glucose + caffeic acid | 90% of the activity with quercetin, UGT73A4 | Beta vulgaris | UDP + caffeoyl 3-O-beta-D-glucoside | - |
? | |
UDP-glucose + chrysin | 70% of the activity with quercetinUGT73A4 | Beta vulgaris | UDP + chrysin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + eriodictyol | 48% of the activity with quercetin, UGT73A4 | Beta vulgaris | UDP + eriodictyol 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + esculetin | 77% of the activity with quercetin, UGT73A4 | Beta vulgaris | UDP + esculetin 6-O-beta-D-glucoside | - |
? | |
UDP-glucose + kaempferol-4'-methylether | 42% of the activity with quercetin, UGT73A4 | Beta vulgaris | UDP + kaempferol-4'-methylether 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + luteolin | 17% of the activity with quercetin, UGT71F1 | Beta vulgaris | UDP + luteolin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + luteolin | as active as with quercetin, UGT73A4 | Beta vulgaris | UDP + luteolin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + myricetin | 80% of the activity with quercetin, 4'-OH glycosylation is preferred over 7-OH glycosylation, UGT73A4 | Beta vulgaris | UDP + myricetin 4'-O-beta-D-glucoside | - |
? | |
UDP-glucose + naringenin | 10% of the activity with quercetin, UGT73A4 | Beta vulgaris | UDP + naringenin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + pinocembrin | 25% of the activity with quercetinUGT73A4 | Beta vulgaris | UDP + pinocembrin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + pinocembrin | 57% of the activity with quercetin, UGT71F1 | Beta vulgaris | UDP + pinocembrin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + quercetagetin | 84% of the activity with quercetin, UGT71F1 | Beta vulgaris | UDP + quercetagetin 3-O-beta-D-glucoside + quercetagetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + quercetagetin | 28% of the activity with quercetin, 4'-OH glycosylation is preferred over 7-OH glycosylation, UGT73A4 | Beta vulgaris | UDP + quercetagetin 4'-O-beta-D-glucoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 54070, calculated from sequence, UGT73A4 | Beta vulgaris |
monomer | 1 * 54390, calculated from sequence, UGT71F1 | Beta vulgaris |
Synonyms | Comment | Organism |
---|---|---|
UGT71F1 | - |
Beta vulgaris |
UGT73A4 | - |
Beta vulgaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
UGT73A4 | Beta vulgaris |
37 | - |
UGT71F1 | Beta vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
UGT73A4 | Beta vulgaris |
7 | 9 | UGT71F1 | Beta vulgaris |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Beta vulgaris | calculated from sequence, MALDI-TOF-MS analysis with molecular masses of tags subtracted, UGT71F1 | - |
5.6 |
Beta vulgaris | calculated from sequence, MALDI-TOF-MS analysis with molecular masses of tags subtracted, UGT73A4 | - |
5.8 |