Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 14 mM, stimulates | Paederia scandens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 41% inhibition | Paederia scandens | |
Co2+ | 1 mM, 95% inhibition | Paederia scandens | |
Cu2+ | 1 mM, 97% inhibition | Paederia scandens | |
dithioerythritol | 10 mM, 50% inhibition | Paederia scandens | |
EDTA | 10 mM, 58% inhibition | Paederia scandens | |
iodoacetamide | 1 mM, 26% inhibition | Paederia scandens | |
iodoacetate | 1 mM, 21% inhibition | Paederia scandens | |
KCl | 10 mM, 30% inhibition | Paederia scandens | |
Mg2+ | 10 mM, 64% inhibition | Paederia scandens | |
Mn2+ | 1 mM, 82% inhibition | Paederia scandens | |
N-ethylmaleimide | 1 mM, 94% inhibition, 14 mM 2-mercaptoethanol restores activity | Paederia scandens | |
p-chloromercuribenzoate | 1 mM, 91% inhibition, 14 mM 2-mercaptoethanol restores activity | Paederia scandens | |
Phenylmercuriacetate | 1 mM, 40% inhibition | Paederia scandens | |
Zn2+ | 1 mM, 98% inhibition | Paederia scandens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00769 | - |
kaempferol | pH 7.5 | Paederia scandens | |
0.01539 | - |
UDP-glucose | pH 7.5, cosubstrate kaempferol | Paederia scandens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for Mg2+, Ca2+ or K+ | Paederia scandens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
gel filtration | Paederia scandens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + a flavonol | Paederia scandens | plays a role in the glycosylation steps of flavonols | UDP + a flavonol 7-O-beta-D-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paederia scandens | - |
var. Mairei | - |
Purification (Comment) | Organism |
---|---|
135.5fold, partial | Paederia scandens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young | Paederia scandens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.63 | - |
pH 7.5 | Paederia scandens |
Storage Stability | Organism |
---|---|
-20°C, partially purified enzyme, 20 mM Tris-HCl, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, 2 days: 15% loss of activity, 8 days: 60% loss of activity | Paederia scandens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | not: rhamnetin, kaempferol 7-O-glucoside, quercetin 7-O-glucoside, ADP-D-glucose, GDP-D-glucose | Paederia scandens | ? | - |
? | |
UDP-alpha-D-glucose + kaempferol | best substrate | Paederia scandens | UDP + kaempferol 7-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + quercetin | 45% of the activity with kaempferol as substrate | Paederia scandens | UDP + quercetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + a flavonol | plays a role in the glycosylation steps of flavonols | Paederia scandens | UDP + a flavonol 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + a flavonol | F 7 GT mediates the transfer of D-glucose exclusively to the 7-hydroxyl group of kaempferol and some flavonols, highly specific for UDP-D-glucose | Paederia scandens | UDP + a flavonol 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + caffeic acid | 14% of the activity with kaempferol as substrate | Paederia scandens | UDP + caffeic acid 4-O-beta-D-glucoside | - |
? | |
UDP-glucose + hydroquinone | 10% of the activity with kaempferol as substrate | Paederia scandens | UDP + hydroquinone O-beta-D-glucoside | - |
? | |
UDP-glucose + isorhamnetin | 88% of the activity with kaempferol as substrate | Paederia scandens | UDP + isorhamnetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + kaempferol 3,4'-dimethylether | 30% of the activity with kaempferol as substrate | Paederia scandens | UDP + kaempferol 3,4'-dimethylether 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + kaempferol 3-O-glucoside | astragalin, 17% of the activity with kaempferol as substrate | Paederia scandens | UDP + kaempferol 3,7-O-beta-D-diglucoside | - |
? | |
UDP-glucose + kaempferol 3-O-rhamnosylglucoside | 11% of the activity with kaempferol as substrate | Paederia scandens | UDP + kaempferol 3-O-rhamnosylglucoside 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + kaempferol 5,7,4'-trimethylether | 9% of the activity with kaempferol as substrate | Paederia scandens | UDP + kaempferol 5,7,4'-trimethylether 3-O-beta-D-glucoside | - |
? | |
UDP-glucose + myricetin | 26% of the activity with kaempferol as substrate | Paederia scandens | UDP + myricetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + p-coumaric acid | 9% of the activity with kaempferol as substrate | Paederia scandens | UDP + (2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid | - |
? | |
UDP-glucose + quercetin 3-O-glucoside | isoquercetin, 32% of the activity with kaempferol as substrate | Paederia scandens | UDP + quercetin 3,7-O-beta-D-diglucoside | - |
? | |
UDP-glucose + rutin | 21% of the activity with kaempferol as substrate | Paederia scandens | UDP + rutin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + scopoletin | 8% of the activity with kaempferol as substrate | Paederia scandens | UDP + scopoletin 7-O-beta-D-glucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
F 7 GT | - |
Paederia scandens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
Tris-HCl and histidine-HCl buffer | Paederia scandens |
8 | - |
imidazole-HCl buffer | Paederia scandens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Paederia scandens | chromatofocusing | - |
4.5 |