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Literature summary for 2.4.1.237 extracted from

  • Ishikura, N.; Yang, Z.Q.
    Multiple forms of flavonol O-glucosyltransferases in young leaves of Euonymus alatus f. ciliato-denatus (1994), Phytochemistry, 36, 1139-1145.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 14 mM, stimulates Euonymus alatus
EDTA 1 mM: slight activation, 10 mM: 36% inhibition Euonymus alatus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 10 mM, 35% inhibition Euonymus alatus
Co2+ 1 mM, 32% inhibition Euonymus alatus
Cu2+ 1 mM, 94% inhibition Euonymus alatus
dithioerythritol 10 mM, 13% inhibition Euonymus alatus
EDTA 10 mM: 36% inhibition, 1 mM: slight activation Euonymus alatus
iodoacetamide 1 mM, 28% inhibition Euonymus alatus
iodoacetate 1 mM, 16% inhibition Euonymus alatus
KCl 1 or 10 mM, 31% inhibition Euonymus alatus
Mg2+ 10 mM, 20% inhibition Euonymus alatus
Mn2+ 10 mM, 37% inhibition Euonymus alatus
N-ethylmaleimide 1 mM, 97% inhibition, 14 mM 2-mercaptoethanol partially restores activity Euonymus alatus
p-chloromercuribenzoate 1 mM, 97% inhibition, 14 mM 2-mercaptoethanol partially restores activity Euonymus alatus
Phenylmercuriacetate 1 mM, 96% inhibition Euonymus alatus
Zn2+ 1 mM, 89% inhibition Euonymus alatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
UDP-glucose pH 7.5, 30°C, cosubstrate kaempferol Euonymus alatus
0.0111
-
kaempferol pH 7.5, 30°C Euonymus alatus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for Mg2+ Euonymus alatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
gel filtration Euonymus alatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Euonymus alatus glycosylation of flavonols ?
-
?

Organism

Organism UniProt Comment Textmining
Euonymus alatus
-
f. Ciliato-dentatus
-

Purification (Commentary)

Purification (Comment) Organism
139.9fold, partial Euonymus alatus

Source Tissue

Source Tissue Comment Organism Textmining
leaf young Euonymus alatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.76
-
pH 7.5, 30°C, kaempferol as substrate Euonymus alatus

Storage Stability

Storage Stability Organism
-20°C, partially purified enzyme, 20 mM Tris-HCl, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, 2 days: 18% loss of activity, 8 days: 64% loss of activity Euonymus alatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information glycosylation of flavonols Euonymus alatus ?
-
?
additional information no or very poor substrates: fisetin, kaempferol 5,7,4’-trimethylether, rhamnetin, kaempferol 7-O-glucoside, quercetin 7-O-glucoside, taxifolin, dihydrokaempferol, ADP-D-glucose, GDP-D-glucose Euonymus alatus ?
-
?
UDP-alpha-D-glucose + kaempferol good substrate Euonymus alatus UDP + kaempferol 7-O-beta-D-glucoside
-
?
UDP-alpha-D-glucose + quercetin 29% of the activity with kaempferol as substrate Euonymus alatus UDP + quercetin 7-O-beta-D-glucoside
-
?
UDP-glucose + 3,5,7-trihydroxyflavanone pinobanksin, 55% of the activity with kaempferol as substrate Euonymus alatus UDP + 3,5,7-trihydroxyflavanone 7-O-beta-D-glucoside
-
?
UDP-glucose + 7,4'-dihydroxyflavonol 53% of the activity with kaempferol as substrate Euonymus alatus UDP + 7,4'-dihydroxyflavonol 7-O-beta-D-glucoside
-
?
UDP-glucose + a flavonol
-
Euonymus alatus UDP + a flavonol 7-O-beta-D-glucoside F7GT mediates the transfer of D-glucose exclusively to the 7-hydroxyl group of kaempferol and some other flavonols, highly specific for UDP-D-glucose as glucosyl donor ?
UDP-glucose + euonymin 16% of the activity with kaempferol as substrate Euonymus alatus UDP + euonymin 7-O-beta-D-glucoside
-
?
UDP-glucose + isoquercitrin 13% of the activity with kaempferol as substrate Euonymus alatus UDP + isoquercitrin 7-O-beta-D-glucoside
-
?
UDP-glucose + isorhamnetin 128% of the activity with kaempferol as substrate Euonymus alatus UDP + isorhamnetin 7-O-beta-D-glucoside
-
?
UDP-glucose + kaempferide 104% of the activity with kaempferol as substrate Euonymus alatus UDP + kaempferide 7-O-beta-D-glucoside
-
?
UDP-glucose + kaempferol 3,4'-di-O-glucoside 12% of the activity with kaempferol as substrate Euonymus alatus UDP + kaempferol 3,7,4'-tri-O-glucoside
-
?
UDP-glucose + kaempferol 3,4'-dimethylether 31% of the activity with kaempferol as substrate Euonymus alatus UDP + kaempferol 3,4'-dimethylether 7-O-beta-D-glucoside
-
?
UDP-glucose + kaempferol 3-O-glucoside astragalin, 20% of the activity with kaempferol as substrate Euonymus alatus UDP + kaempferol 3,7-O-diglucoside
-
?
UDP-glucose + myricetin 36% of the activity with kaempferol as substrate Euonymus alatus UDP + myricetin 7-O-beta-D-glucoside
-
?
UDP-glucose + quercetin 5-methylether 13% of the activity with kaempferol as substrate Euonymus alatus UDP + quercetin 5-methylether 7-O-beta-D-glucoside
-
?

Subunits

Subunits Comment Organism
monomer 1 * 48000, SDS-PAGE Euonymus alatus

Synonyms

Synonyms Comment Organism
F7GT
-
Euonymus alatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Euonymus alatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
Tris-HCl buffer Euonymus alatus

pI Value

Organism Comment pI Value Maximum pI Value
Euonymus alatus chromatofocusing
-
4.5