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Literature summary for 2.4.1.234 extracted from

  • Miller, K.D.; Strommer, J.; Taylor, L.P.
    Conservation in divergent solanaceous species of the unique gene structure and enzyme activity of a gametophytically-expressed flavonol 3-O-galactosyltransferase (2002), Plant Mol. Biol., 48, 233-242.
    View publication on PubMed
Search for more literature for 2.4.1.234

Cloned(Commentary)

Cloned (Comment) Organism
F3GalTase cDNA from V26 plants, expression in Escherichia coli TOP10, sequencing, F3galtase gene structure, genomic organization Petunia x hybrida
F3galtase gene structure Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by 1% Triton X-100 Petunia x hybrida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Petunia x hybrida F3GalTase glycosylates the flavonols required for pollen germination, F3galtase gene regulation ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Triton X-100 1%, no reduction of activity during extraction Petunia x hybrida

Organism

Organism UniProt Comment Textmining
Nicotiana alata
-
 F3GalTase activity is specific to the Solanaceae
-
Nicotiana tabacum
-
 cv. Xanthi, F3GalTase activity is specific to the Solanaceae
-
Petunia x hybrida Q9SBQ8 V26, V10 and W78 plants, F3GalTase activity is specific to the Solanaceae
-
Solanum lycopersicum
-
 F3GalTase activity is specific to the Solanaceae
-
Solanum tuberosum
-
 F3GalTase activity is specific to the Solanaceae
-

Source Tissue

Source Tissue Comment Organism Textmining
flower stage 6-9, corolla Petunia x hybrida
-
gametophyte gametophytically-expressed F3GalTase Solanum tuberosum
-
gametophyte gametophytically-expressed F3GalTase Nicotiana tabacum
-
gametophyte gametophytically-expressed F3GalTase Solanum lycopersicum
-
gametophyte gametophytically-expressed F3GalTase Petunia x hybrida
-
gametophyte gametophytically-expressed F3GalTase Nicotiana alata
-
pollen lower F3GalTase activity than in Petunia hybrida pollen Solanum tuberosum
-
pollen lower F3GalTase activity than in Petunia hybrida pollen Nicotiana tabacum
-
pollen lower F3GalTase activity than in Petunia hybrida pollen Solanum lycopersicum
-
pollen lower F3GalTase activity than in Petunia hybrida pollen Nicotiana alata
-
pollen pollen-specific enzyme Petunia x hybrida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Solanum tuberosum
additional information
-
-
 Nicotiana tabacum
additional information
-
-
 Solanum lycopersicum
additional information
-
-
 Petunia x hybrida
additional information
-
-
 Nicotiana alata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information F3GalTase glycosylates the flavonols required for pollen germination, F3galtase gene regulation Petunia x hybrida ?
-
 ?
UDP-galactose + flavonol
-
 Solanum tuberosum UDP + flavonol 3-O-beta-D-galactoside
-
 ?
UDP-galactose + flavonol
-
 Nicotiana tabacum UDP + flavonol 3-O-beta-D-galactoside
-
 ?
UDP-galactose + flavonol
-
 Solanum lycopersicum UDP + flavonol 3-O-beta-D-galactoside
-
 ?
UDP-galactose + flavonol
-
 Nicotiana alata UDP + flavonol 3-O-beta-D-galactoside
-
 ?
UDP-galactose + flavonol highly specific for flavonols and UDP-galactose Petunia x hybrida UDP + flavonol 3-O-beta-D-galactoside
-
 ?
UDP-galactose + kaempferol
-
 Petunia x hybrida UDP + kaempferol 3-O-beta-D-galactoside
-
 ?
UDP-galactose + quercetin
-
 Petunia x hybrida UDP + quercetin 3-O-beta-D-galactoside
-
 ?