Any feedback?
Literature summary for 2.4.1.231 extracted from
- Trehalose phosphorylase from Pleurotus ostreatus: characterization and stabilization by covalent modification, and application for the synthesis of alpha,alpha-trehalose (2007), J. Biotechnol., 129, 140-150.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | sucrose is converted to alpha,alpha-trehalose in about 60% yield using a coupled enzyme system composed of sucrose phosphorylase from Leuconostoc mesenteroides, glucose isomerase from Streptomyces murinus and the appropriately stabilized PoTPase | Pleurotus ostreatus |
General Stability
| General Stability | Organism |
|---|---|
| unstable protein, half-life of no more than approximately 1.3 h in 20mM MES buffer, 25°C, pH 7.0. The enzyme can be stabilized on addition of alpha,alpha-trehalose (300 mM) which yields a half-life of 11.5 h. In the presence of 20% (w/v) glycerol, and 9 and 26% (w/v) PEG-4000, half-life is 6.5 h, 16 h and 70 h, respectively. Large improvements in stability by covalent protein modification with activated derivatives of PEG-5000 | Pleurotus ostreatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| vanadate | - |
Pleurotus ostreatus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
phosphate | - |
Pleurotus ostreatus | |
| 3.5 | - |
phosphate | 30°C, pH 6.8 | Pleurotus ostreatus | |
| 4.1 | - |
alpha-D-glucose 1-phosphate | - |
Pleurotus ostreatus | |
| 4.1 | - |
alpha-D-glucose 1-phosphate | 30°C, pH 6.8 | Pleurotus ostreatus | |
| 40 | - |
alpha-D-glucose | - |
Pleurotus ostreatus | |
| 40 | - |
alpha-D-glucose | 30°C, pH 6.8 | Pleurotus ostreatus | |
| 79 | - |
alpha,alpha-trehalose | - |
Pleurotus ostreatus | |
| 79 | - |
alpha,alpha-trehalose | 30°C, pH 6.8 | Pleurotus ostreatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS PAGE | Pleurotus ostreatus |
| 58300 | - |
gel filtration | Pleurotus ostreatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pleurotus ostreatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pleurotus ostreatus |
| trehalose phosphorylase from the basidiomycete Pleurotus ostreatus (PoTPase) is isolated from fungal fruit bodies through 500-fold purification with a yield of 44% | Pleurotus ostreatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit body | - |
Pleurotus ostreatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pleurotus ostreatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-dehydro-D-glucose + alpha-D-glucose 1-phosphate | - |
Pleurotus ostreatus | ? + phosphate | - |
? | |
| 2-deoxy-D-glucose + alpha-D-glucose 1-phosphate | 8% of the activity with alpha-D-glucose | Pleurotus ostreatus | ? | - |
r | |
| 2-deoxy-D-glucose + alpha-D-glucose 1-phosphate | - |
Pleurotus ostreatus | ? + phosphate | - |
? | |
| 2-fluoro-D-glucose + alpha-D-glucose 1-phosphate | 15% of the activity with alpha-D-glucose | Pleurotus ostreatus | ? | - |
r | |
| 2-fluoro-D-glucose + alpha-D-glucose 1-phosphate | - |
Pleurotus ostreatus | ? + phosphate | - |
? | |
| 2-keto-D-glucose + alpha-D-glucose 1-phosphate | 50% of the activity with alpha-D-glucose | Pleurotus ostreatus | ? | - |
r | |
| alpha,alpha-trehalose + arsenate | 30% of the activity with phosphate | Pleurotus ostreatus | alpha-D-glucose 1-arsenate + alpha-D-glucose | - |
? | |
| alpha,alpha-trehalose + arsenate | - |
Pleurotus ostreatus | alpha-D-glucose + alpha-D-glucose 1-arsenate | - |
r | |
| alpha,alpha-trehalose + phosphate | - |
Pleurotus ostreatus | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
| alpha-D-glucose + alpha-D-glucose 1-phosphate | beta-D-glucose 1-phosphate is not turned over by the enzyme | Pleurotus ostreatus | alpha,alpha-trehalose + phosphate | - |
r | |
| D-mannose + alpha-D-glucose 1-phosphate | 3% of the activity with alpha-D-glucose | Pleurotus ostreatus | ? | - |
r | |
| D-mannose + alpha-D-glucose 1-phosphate | - |
Pleurotus ostreatus | ? + phosphate | - |
? | |
| additional information | no measurable activity is found with D-arabinose, D-fructose, D-fucose, D-galactose, D-lyxose, D-mannoheptaose, D-ribose and D-xylose | Pleurotus ostreatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 55000, SDS-PAGE | Pleurotus ostreatus |
| monomer | gel filtration | Pleurotus ostreatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PoTPase | - |
Pleurotus ostreatus |
| trehalose phosphorylase | - |
Pleurotus ostreatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pleurotus ostreatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
PoTPase has a half life of approximately 1 h at 30°C | Pleurotus ostreatus |
| 30 | - |
half-life: approximately 1 h in absence of stabilizing compound, 11.5 h in presence of 300 mM alpha,alpha-trehalose, 6.5 h in presence of 20% w/v glycerol, 70 h in presence of 26% w/v polyethylenglycol. Covalent modification of PoTPase with activated derivatives of PEG 5000 increases the stability by up to 600fold | Pleurotus ostreatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.27 | - |
2-deoxy-D-glucose | - |
Pleurotus ostreatus | |
| 0.42 | - |
D-mannose | - |
Pleurotus ostreatus | |
| 0.89 | - |
2-fluoro-D-glucose | - |
Pleurotus ostreatus | |
| 1.15 | - |
arsenate | - |
Pleurotus ostreatus | |
| 4.5 | - |
2-dehydro-D-glucose | - |
Pleurotus ostreatus | |
| 16 | - |
alpha-D-glucose 1-phosphate | - |
Pleurotus ostreatus | |
| 16 | - |
alpha-D-glucose 1-phosphate | 30°C, pH 6.8 | Pleurotus ostreatus | |
| 16.8 | - |
alpha-D-glucose | - |
Pleurotus ostreatus | |
| 16.8 | - |
alpha-D-glucose | 30°C, pH 6.8 | Pleurotus ostreatus | |
| 17.6 | - |
phosphate | - |
Pleurotus ostreatus | |
| 17.6 | - |
phosphate | 30°C, pH 6.8 | Pleurotus ostreatus | |
| 18.3 | - |
alpha,alpha-trehalose | - |
Pleurotus ostreatus | |
| 18.3 | - |
alpha,alpha-trehalose | 30°C, pH 6.8 | Pleurotus ostreatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 6.4 | synthesis of alpha,alpha-trehalose | Pleurotus ostreatus |
| 6.2 | - |
alpha, alpha-trehalose synthesis | Pleurotus ostreatus |
| 6.8 | - |
assay at | Pleurotus ostreatus |
| 6.8 | - |
alpha, alpha-trehalose phosphorolysis | Pleurotus ostreatus |
| 6.8 | 7.2 | phosphorolysis | Pleurotus ostreatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.8 | pH 5.5: about 75% of maximal activity, pH 7.8: about 80% of maxuimal activity, phosphorolysis | Pleurotus ostreatus |
| 5.5 | 6.8 | pH 5.5: about 80% of maximal activity, pH 75% of maximal activity, synthesis of alpha,alpha-trehalose | Pleurotus ostreatus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
vanadate | - |
Pleurotus ostreatus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
