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Literature summary for 2.4.1.230 extracted from

  • Jung, J.H.; Seo, D.H.; Holden, J.F.; Park, C.S.
    Identification and characterization of an archaeal kojibiose catabolic pathway in the hyperthermophilic Pyrococcus sp. strain ST04 (2014), J. Bacteriol., 196, 1122-1131.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Escherichia coli Pyrococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
MnCl2 1 mM, 34% loss of activity Pyrococcus sp.
additional information 1 mM EDTA does not effect activity Pyrococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.34
-
phosphate pH 6.0, 90°C Pyrococcus sp.
2.5 3 2-alpha-D-glucosyl-D-glucose pH 6.0, 90°C Pyrococcus sp.
36.8
-
D-glucose pH 6.0, 90°C Pyrococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
CdCl2 1 mM, elevates hydrolysis activity by 33% Pyrococcus sp.
CuCl2 1 mM, elevates hydrolysis activity by 40% Pyrococcus sp.
additional information the enzyme does not require metal ions Pyrococcus sp.
ZnCl2 1 mM, elevates hydrolysis activity by 36% Pyrococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
x * 80000, SDS-PAGE Pyrococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-alpha-D-glucosyl-D-glucose + phosphate Pyrococcus sp. 2-alpha-D-glucosyl-D-glucose i.e. kojibiose. The enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea D-glucose + beta-D-glucose 1-phosphate
-
?
2-alpha-D-glucosyl-D-glucose + phosphate Pyrococcus sp. ST04 2-alpha-D-glucosyl-D-glucose i.e. kojibiose. The enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea D-glucose + beta-D-glucose 1-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus sp. I3RFR4
-
-
Pyrococcus sp. ST04 I3RFR4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pyrococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.7
-
pH 6.0, 80°C, substrate: kojibiose Pyrococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-alpha-D-glucosyl-D-glucose + phosphate 2-alpha-D-glucosyl-D-glucose i.e. kojibiose. The enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea Pyrococcus sp. D-glucose + beta-D-glucose 1-phosphate
-
?
2-alpha-D-glucosyl-D-glucose + phosphate 2-alpha-D-glucosyl-D-glucose i.e. kojibiose Pyrococcus sp. D-glucose + beta-D-glucose 1-phosphate
-
r
2-alpha-D-glucosyl-D-glucose + phosphate 2-alpha-D-glucosyl-D-glucose i.e. kojibiose. The enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea Pyrococcus sp. ST04 D-glucose + beta-D-glucose 1-phosphate
-
?
2-alpha-D-glucosyl-D-glucose + phosphate 2-alpha-D-glucosyl-D-glucose i.e. kojibiose Pyrococcus sp. ST04 D-glucose + beta-D-glucose 1-phosphate
-
r
3-O-alpha-D-glucosyl-D-glucose + phosphate 1-alpha-D-glucosyl-D-glucose i.e. nigerose, at 0.23% of the activity with kojibiose Pyrococcus sp. D-glucose + beta-D-glucose 1-phosphate
-
?
3-O-alpha-D-glucosyl-D-glucose + phosphate 1-alpha-D-glucosyl-D-glucose i.e. nigerose, at 0.23% of the activity with kojibiose Pyrococcus sp. ST04 D-glucose + beta-D-glucose 1-phosphate
-
?
additional information no activity with trehalose or cellobiose. Activity with maltose or isomaltose es less than 0.001% of the activity with kojibiose Pyrococcus sp. ?
-
?
additional information no activity with trehalose or cellobiose. Activity with maltose or isomaltose es less than 0.001% of the activity with kojibiose Pyrococcus sp. ST04 ?
-
?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Pyrococcus sp.

Synonyms

Synonyms Comment Organism
kojibiose phosphorylase
-
Pyrococcus sp.
PsKP
-
Pyrococcus sp.
Py04_1502 locus name Pyrococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Pyrococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
20 min, stable Pyrococcus sp.
95
-
half-life: 71 h Pyrococcus sp.
100
-
2 h, over 40% of the activity remains. Half-life: 1.9 h Pyrococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
37.7
-
phosphate pH 6.0, 90°C Pyrococcus sp.
37.7
-
2-alpha-D-glucosyl-D-glucose pH 6.0, 90°C Pyrococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Pyrococcus sp.
6
-
assay at Pyrococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 6 over 90% of maximal activity at pH 4.0 and at pH 6.0 Pyrococcus sp.

General Information

General Information Comment Organism
physiological function the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea Pyrococcus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
14.9
-
2-alpha-D-glucosyl-D-glucose pH 6.0, 90°C Pyrococcus sp.
28.1
-
phosphate pH 6.0, 90°C Pyrococcus sp.