Cloned (Comment) | Organism |
---|---|
heterologous expression in Escherichia coli | Pyrococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MnCl2 | 1 mM, 34% loss of activity | Pyrococcus sp. | |
additional information | 1 mM EDTA does not effect activity | Pyrococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.34 | - |
phosphate | pH 6.0, 90°C | Pyrococcus sp. | |
2.5 | 3 | 2-alpha-D-glucosyl-D-glucose | pH 6.0, 90°C | Pyrococcus sp. | |
36.8 | - |
D-glucose | pH 6.0, 90°C | Pyrococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CdCl2 | 1 mM, elevates hydrolysis activity by 33% | Pyrococcus sp. | |
CuCl2 | 1 mM, elevates hydrolysis activity by 40% | Pyrococcus sp. | |
additional information | the enzyme does not require metal ions | Pyrococcus sp. | |
ZnCl2 | 1 mM, elevates hydrolysis activity by 36% | Pyrococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, SDS-PAGE | Pyrococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-alpha-D-glucosyl-D-glucose + phosphate | Pyrococcus sp. | 2-alpha-D-glucosyl-D-glucose i.e. kojibiose. The enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea | D-glucose + beta-D-glucose 1-phosphate | - |
? | |
2-alpha-D-glucosyl-D-glucose + phosphate | Pyrococcus sp. ST04 | 2-alpha-D-glucosyl-D-glucose i.e. kojibiose. The enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea | D-glucose + beta-D-glucose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus sp. | I3RFR4 | - |
- |
Pyrococcus sp. ST04 | I3RFR4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pyrococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.7 | - |
pH 6.0, 80°C, substrate: kojibiose | Pyrococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-alpha-D-glucosyl-D-glucose + phosphate | 2-alpha-D-glucosyl-D-glucose i.e. kojibiose. The enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea | Pyrococcus sp. | D-glucose + beta-D-glucose 1-phosphate | - |
? | |
2-alpha-D-glucosyl-D-glucose + phosphate | 2-alpha-D-glucosyl-D-glucose i.e. kojibiose | Pyrococcus sp. | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
2-alpha-D-glucosyl-D-glucose + phosphate | 2-alpha-D-glucosyl-D-glucose i.e. kojibiose. The enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea | Pyrococcus sp. ST04 | D-glucose + beta-D-glucose 1-phosphate | - |
? | |
2-alpha-D-glucosyl-D-glucose + phosphate | 2-alpha-D-glucosyl-D-glucose i.e. kojibiose | Pyrococcus sp. ST04 | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
3-O-alpha-D-glucosyl-D-glucose + phosphate | 1-alpha-D-glucosyl-D-glucose i.e. nigerose, at 0.23% of the activity with kojibiose | Pyrococcus sp. | D-glucose + beta-D-glucose 1-phosphate | - |
? | |
3-O-alpha-D-glucosyl-D-glucose + phosphate | 1-alpha-D-glucosyl-D-glucose i.e. nigerose, at 0.23% of the activity with kojibiose | Pyrococcus sp. ST04 | D-glucose + beta-D-glucose 1-phosphate | - |
? | |
additional information | no activity with trehalose or cellobiose. Activity with maltose or isomaltose es less than 0.001% of the activity with kojibiose | Pyrococcus sp. | ? | - |
? | |
additional information | no activity with trehalose or cellobiose. Activity with maltose or isomaltose es less than 0.001% of the activity with kojibiose | Pyrococcus sp. ST04 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Pyrococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
kojibiose phosphorylase | - |
Pyrococcus sp. |
PsKP | - |
Pyrococcus sp. |
Py04_1502 | locus name | Pyrococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Pyrococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
20 min, stable | Pyrococcus sp. |
95 | - |
half-life: 71 h | Pyrococcus sp. |
100 | - |
2 h, over 40% of the activity remains. Half-life: 1.9 h | Pyrococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37.7 | - |
phosphate | pH 6.0, 90°C | Pyrococcus sp. | |
37.7 | - |
2-alpha-D-glucosyl-D-glucose | pH 6.0, 90°C | Pyrococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Pyrococcus sp. |
6 | - |
assay at | Pyrococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | over 90% of maximal activity at pH 4.0 and at pH 6.0 | Pyrococcus sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea | Pyrococcus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.9 | - |
2-alpha-D-glucosyl-D-glucose | pH 6.0, 90°C | Pyrococcus sp. | |
28.1 | - |
phosphate | pH 6.0, 90°C | Pyrococcus sp. |