Cloned (Comment) | Organism |
---|---|
- |
Thermoanaerobacter brockii |
Protein Variants | Comment | Organism |
---|---|---|
D177N | 106% of wild-type activity | Thermoanaerobacter brockii |
D271N | 69% of wild-type activity | Thermoanaerobacter brockii |
D340N | 89% of wild-type activity | Thermoanaerobacter brockii |
D362N | complete loss of enzymic activity | Thermoanaerobacter brockii |
D459N | increase in Km-values for kojibiose, beta-D-glucose 1-phosphate and glucose | Thermoanaerobacter brockii |
E284Q | 103% of wild-type activity | Thermoanaerobacter brockii |
E642Q | complete loss of enzymic activity | Thermoanaerobacter brockii |
K114Q | 85% of wild-type activity | Thermoanaerobacter brockii |
K403Q | 73% of wild-type activity | Thermoanaerobacter brockii |
K614Q | complete loss of enzymic activity | Thermoanaerobacter brockii |
K749Q | 78% of wild-type activity | Thermoanaerobacter brockii |
R137Q | 61% of wild-type activity | Thermoanaerobacter brockii |
R33Q | 31% of wild-type activity | Thermoanaerobacter brockii |
R476Q | 50% of wild-type activity | Thermoanaerobacter brockii |
R48Q | 62% of wild-type activity | Thermoanaerobacter brockii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.75 | - |
phosphate | wild-type, pH 5.5, 60°C | Thermoanaerobacter brockii | |
0.79 | - |
beta-D-glucose 1-phosphate | wild-type, pH 5.5, 60°C | Thermoanaerobacter brockii | |
0.8 | - |
kojibiose | wild-type, pH 5.5, 60°C | Thermoanaerobacter brockii | |
1.11 | - |
phosphate | mutant D459N, pH 5.5, 60°C | Thermoanaerobacter brockii | |
3.84 | - |
beta-D-glucose | wild-type, pH 5.5, 60°C | Thermoanaerobacter brockii | |
5.21 | - |
kojibiose | mutant D459N, pH 5.5, 60°C | Thermoanaerobacter brockii | |
8.57 | - |
beta-D-glucose 1-phosphate | mutant D459N, pH 5.5, 60°C | Thermoanaerobacter brockii | |
25.4 | - |
beta-D-glucose | mutant D459N, pH 5.5, 60°C | Thermoanaerobacter brockii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter brockii | - |
strain ATCC35047, expression in Escherichia coli | - |
Thermoanaerobacter brockii ATCC 35047 | - |
strain ATCC35047, expression in Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + beta-D-glucose 1-phosphate | - |
Thermoanaerobacter brockii | 2-alpha-D-glucosyl-D-glucose + phosphate | - |
r | |
beta-D-glucose + beta-D-glucose 1-phosphate | - |
Thermoanaerobacter brockii ATCC 35047 | 2-alpha-D-glucosyl-D-glucose + phosphate | - |
r | |
kojibiose + phosphate | - |
Thermoanaerobacter brockii | beta-D-glucose-1-phosphate + D-glucose | - |
r | |
kojibiose + phosphate | - |
Thermoanaerobacter brockii ATCC 35047 | beta-D-glucose-1-phosphate + D-glucose | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermoanaerobacter brockii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Thermoanaerobacter brockii |