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Literature summary for 2.4.1.229 extracted from

  • van der Wel, H.; Johnson, J.M.; Xu, Y.; Karunaratne, C.V.; Wilson, K.D.; Vohra, Y.; Boons, G.J.; Taylor, C.M.; Bendiak, B.; West, C.M.
    Requirements for Skp1 processing by cytosolic prolyl 4(trans)-hydroxylase and alpha-N-acetylglucosaminyltransferase enzymes involved in O2 signaling in dictyostelium (2011), Biochemistry, 50, 1700-1713.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.229

Cloned(Commentary)

Cloned (Comment) Organism
cloning of a hybrid Gnt1, the coding sequence is generated from the 16 amino acid exon 1 of Dictyostelium discoideum Gnt1 and exon 2 of Dictyostelium purpureum Gnt1, and expression in Escherichia coli as His-tagged protein Dictyostelium discoideum
cloning of a hybrid Gnt1, the coding sequence is generated from the 16 amino acid exon 1 of Dictyostelium discoideum Gnt1 and exon 2 of Dictyostelium purpureum Gnt1, and expression in Escherichia coli as His-tagged protein Dictyostelium purpureum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline Dictyostelium discoideum
-
 UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline Dictyostelium purpureum
-
 UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
-
-
Dictyostelium purpureum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged hybrid Gnt1, formed of Dictyostelium discoideum Gnt1/Dictyostelium purpureum Gnt1, from Escherichia coli by nickel affinity chromatography Dictyostelium purpureum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity of recombinant Gnt1 hydrid enzyme, although synthetic 13-mer and 23-mer hydroxyproline peptides are substrates than Skyp1A, their activities are much lower even at 5000fold higher concentration, the 40-mer hydroxyproline peptide is also a poor substrate, overview Dictyostelium purpureum ?
-
 ?
additional information substrate specificity of recombinant Gnt1 hydrid enzyme, although synthetic 13-mer and 23-mer hydroxyproline peptides are substrates, their activities are much lower even at 5000fold higher concentration, the 40-mer hydroxyproline peptide is also a poor substrate, overview Dictyostelium discoideum ?
-
 ?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
-
 Dictyostelium discoideum UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
-
 Dictyostelium purpureum UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline transfer to the hydroxyproline, at the Thr-Pro143 dipeptide, preceded by a coil and succeeded by an alpha-helix in Skp1 Dictyostelium discoideum UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline transfer to the hydroxyproline, at the Thr-Pro143 dipeptide, preceded by a coil and succeeded by an alpha-helix in Skp1 Dictyostelium purpureum UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?

Synonyms

Synonyms Comment Organism
GnT1
-
 Dictyostelium discoideum
GnT1
-
 Dictyostelium purpureum
hydroxyproline-capping enzyme
-
 Dictyostelium discoideum
hydroxyproline-capping enzyme
-
 Dictyostelium purpureum

General Information

General Information Comment Organism
metabolism a hypoxia inducible factor-alpha (HIFR) type prolyl 4-hydroxylase, P4H1, and an alpha-N-acetylglucosaminyltransferase Gnt1, sequentially modify proline 143 of Skp1, a subunit of the Skp1/Cullin/F-box protein, SCF, class of E3 ubiquitin ligases Dictyostelium discoideum
metabolism a hypoxia inducible factor-alpha (HIFR) type prolyl 4-hydroxylase, P4H1, and an alpha-N-acetylglucosaminyltransferase Gnt1, sequentially modify proline 143 of Skp1, a subunit of the Skp1/Cullin/F-box protein, SCF, class of E3 ubiquitin ligases Dictyostelium purpureum
additional information Gnt1 from Dictyostelium discoideum contains a stretch of 80 Asn residues and other Asn-rich inserts Dictyostelium discoideum
additional information Gnt1 from Dictyostelium purureum lacks a stretch of 80 Asn residues and other Asn-rich inserts Dictyostelium purpureum